实用医学杂志2023，Vol.39Issue(23)：3027-3033,7.DOI:10.3969/j.issn.1006-5725.2023.23.002
白细胞分化抗原74和CXC趋化因子配体9阳性巨噬细胞亚群在大鼠肝移植排斥反应中的作用
Changes and role of CD74 and CXCL9 positive macrophage subsets in rejection of rat liver transplantation
摘要
Abstract
Objective To explore the classification and changes of macrophage subsets in liver transplant rejection.Methods Rat liver transplantation model were established and divided into immune tolerance group(B-B),where the liver of BN rat donors was transplanted to BN rat recipients,and immune rejection group(L-B),in which the liver of Lewis rat donors was transplanted to BN rat recipients.Single-cell RNA sequencing and high-throughput RNA sequencing were used to distinguish the macrophage subsets of rat liver transplantation,and to find differential gene in rejection reactions.Immunohistochemistry was used to determine the changes and distribu-tion of protein expression and cell subsets.Results CD68 positive macrophages were higher in the rejection group than that in the tolerance group(P<0.05),and macrophages could be divided into 9 subsets.During the rejection reaction,the CXC chemokine ligand 9(CXCL9)in the 8th subsets of macrophages was significantly increased,while the gene for white blood cell differentiation antigen 74(CD74)in the 5th subsets was significantly increased(P<0.05).CD74 ranked first in the differential gene synthesis of macrophages during rejection,followed by CXCL9.Compared with the tolerance group,a large number of CD74 positive macrophages were observed in the hepatic portal area of the rejection group,and the infiltration of CD74 positive macrophages in the hepatic sinuses was also significantly increased(P<0.05),while a large number of CXCL9 positive macrophages were observed in the hepatic portal area and hepatic sinuses of the rejection group,especially in the portal area(P<0.05),and CD14 positive cells were significantly increased(P<0.05).Conclusions The CD74 positive macrophage subsets and CXCL9 positive macrophage subsets may be key subgroups in promoting liver transplant rejection,improving the mechanism of macrophage action in liver transplant rejection.关键词
肝移植/排斥反应/巨噬细胞/白细胞分化抗原74/CXC趋化因子配体9Key words
liver transplantation/rejection/macrophages/cluster of differentiation 74/C-X-C motif chemokine ligand 9分类
医药卫生引用本文复制引用
魏思东,陈凯歌,张继翔,轩娟娟,王耀权,苗舜,赵开心,王维伟,陈国勇..白细胞分化抗原74和CXC趋化因子配体9阳性巨噬细胞亚群在大鼠肝移植排斥反应中的作用[J].实用医学杂志,2023,39(23):3027-3033,7.基金项目
国家自然科学基金资助项目(编号:U2004124) （编号:U2004124）
河南省科技攻关计划项目(编号:212102310717) （编号:212102310717）
