实用医学杂志2023，Vol.39Issue(23)：3043-3050,8.DOI:10.3969/j.issn.1006-5725.2023.23.004
LncRNA DSCAM-AS1调节miR-150-5p/BRAF轴对甲状腺癌细胞恶性生物学行为的影响
Impact of LncRNA DSCAM-AS1 on the malignant biological behaviors of thyroid cancer cells by regulat-ing the miR-150-5p/BRAF axis
摘要
Abstract
Objective To investigate the impact of lncRNA DSCAM-AS1 on the malignant biological behaviors of thyroid cancer(TC)cells by regulating the miR-150-5p/BRAF axis.Methods The expression of DSCAM-AS1 in TC cells was detected by qRT-PCR,and the best intervention cell line was screened.;the relationship between DSCAM-AS1,BRAF and miR-150-5p targeting regulation was verified by FISH,pull down and double Luciferase reporter gene experiment;The proliferation,migration and invasion of SW579 cells were detected;Western blot was applied to detect the expression of BRAF,E-Cadherin,and vimentin proteins;the tumor formation experiment in mice was applied to verify the effect of DSCAM-AS1 on TC tumor growth.Results DSCAM-AS1 was highly expressed in TC tissue and cells(P<0.05);There is a targeted regulation relationship between DSCAM-AS1,BRAF and miR-150-5p;inhibition of DSCAM-AS1 expression or overexpression of miR-150-5p obviously inhibited the proliferation,migration,invasion,and EMT of SW579 cells(P<0.05);Inhibition of miR-150-5p expression or overexpression of BRAF reversed the inhibitory effect of inhibition of DSCAM-AS1 expression or overexpression of miR-150-5p on the malignant behavior of SW579 cells(P<0.05);in vivo experiments showed that inhibiting the expression of DSCAM-AS1 obviously inhibited the growth of transplanted tumors in mice(P<0.05).Conclusion DSCAM-AS1 is up-regulated in TC cells,inhibiting the expression of LncRNA DSCAM-AS1 can inhibit the malignant progression of TC by regulating the miR-150-5p/BRAF signaling axis.关键词
lncRNA DSCAM-AS1/miR-150-5p/BRAF轴/甲状腺癌/增殖/迁移/侵袭Key words
lncRNA DSCAM-AS1/miR-150-5p/BRAF axis/thyroid cancer/proliferation/migra-tion/invasion分类
医药卫生引用本文复制引用
彭云,温美玲,吕云霞,陈万志,何春,俞建平,丁振罗..LncRNA DSCAM-AS1调节miR-150-5p/BRAF轴对甲状腺癌细胞恶性生物学行为的影响[J].实用医学杂志,2023,39(23):3043-3050,8.基金项目
国家自然科学基金项目(编号:82204879) （编号:82204879）
江西省卫生健康委员会科技计划项目(编号:202311874) （编号:202311874）
江西省卫生健康委员会科技计划项目(编号:20194051) （编号:20194051）
