生态环境学报2023，Vol.32Issue(10)：1719-1731,13.DOI:10.16258/j.cnki.1674-5906.2023.10.001
针阔人工混交林及其纯林对土壤微生物碳循环功能基因丰度的影响
Effect of Monoculture and Mixed Plantation with Coniferous and Broadleaved Tree Species on Soil Microbial Carbon Cycle Functional Gene Abundance
摘要
Abstract
Exploring the abundance of carbon cycle functional genes in soil microorganisms is important for understanding the mechanism of soil carbon cycling.However,the response characteristics of soil microbial carbon cycle functional gene abundance to different plantation types are still unclear.Based on the metagenomic sequencing data,soil physicochemical properties,and organic carbon components of soil samples from different soil layers(0-20,20-40,40-60 cm)under the mixed species plantation with Pinus massoniana and Erythrophleum fordii,as well as their pure plantations in south subtropical China,the differences in microbial functional gene abundance related to soil carbon cycling(carbon fixation,carbon degradation and methane metabolism)between different soil layers of different stands,and the dominant soil environmental factors were analyzed.The results showed that the abundance of soil microbial carbon fixation functional genes(rcbL,MUT and PCCA)in the Pinus massoniana stand was significantly higher than that in the other two stands.This difference was attributed to higher soil total phosphorus(TP)content and significantly lower contents of microbial biomass carbon(MBC),easily oxidizable organic carbon(EOC),particulate organic carbon(POC),and recalcitrant organic carbon(ROC)(P<0.05).The abundance of carbon degradation functional genes(MAN2C1 and bglB)was significantly higher in the Pinus massoniana stand than that in mixed plantation.These differences were mainly affected by the low content of soil organic carbon(SOC),MBC,dissolved organic carbon(DOC),EOC and ROC in Pinus massoniana plantation.The highest abundance of methane metabolism functional genes(pmoA-amoA,pmoB-amoB and pmoC-amoC)was observed in the Pinus massoniana plantation,which was related to significant negative effects of SOC,MBC,DOC,EOC,and ROC.Additionally,the abundance of microbial carbon cycle functional genes in all three planted forests increased with increasing soil depth,mainly due to the decrease in SOC,C/N ratio,MBC,DOC,EOC and ROC contents.Overall,the Pinus massoniana stand exhibited a relatively high potential for soil microbial carbon cycling,while the soil microbial carbon cycle potential in all three stands increased with soil depth.The composition of soil organic carbon was identified as an important factor regulating the difference in functional gene abundance of soil microbial carbon cycling in these plantations.关键词
土壤微生物碳循环功能基因/土壤有机碳组分/宏基因组测序/人工林/南亚热带Key words
soil microbial carbon cycle functional genes/soil organic carbon components/metagenomic sequencing/planted forest/south subtropical China分类
农业科技引用本文复制引用
秦佳琪,肖指柔,明安刚,朱豪,滕金倩,梁泽丽,陶怡,覃林..针阔人工混交林及其纯林对土壤微生物碳循环功能基因丰度的影响[J].生态环境学报,2023,32(10):1719-1731,13.基金项目
国家自然科学基金项目(31560109 （）
32071764) （）
广西自然科学基金项目(2020GXNSFAA297208) （2020GXNSFAA297208）
