山西医科大学学报2023，Vol.54Issue(11)：1449-1454,6.DOI:10.13753/j.issn.1007-6611.2023.11.003
扶正利湿抗癌方对LNCaP细胞中PSMA表达及p38 MAPK通路的影响
Effect of Fuzheng Lishi Kang'ai decoction on PSMA expression and p38 MAPK pathway in LNCaP cells
摘要
Abstract
Objective To investigate the effects of Fuzheng Lishi Kang'ai decoction on the expression of prostate-specific membrane antigen(PSMA)and the p38 MAPK pathway in human prostate cancer LNCaP cells.Methods SD rats were randomly divided into four groups:control group,low-dose group[12.5 g/(kg·d)],medium-dose group[25 g/(kg·d)],and high-dose group[50 g/(kg·d)].Serum containing different concentrations of drug in each group was extracted and co-cultured with LNCaP cells.The expression of PSMA in LNCaP cells was detected using the 18F-PSMA1007 probe.The expressions of p38 MAPK,p-p38 MAPK,Bax,Caspase-3,P53,and Bcl-2 proteins related to the p38 MAPK pathway in LNCaP cells were detected using Western blot.The proliferation inhibi-tion of LNCaP cells was observed using the CCK-8 assay.The apoptosis of LNCaP cells was analyzed using flow cytometry.Results Compared with control group,the expression of PSMA was decreased in low-dose group,the expressions of p-p38 MAPK,Bax,Caspase-3,and P53 proteins were increased,the expression of Bcl-2 protein was decreased,the proliferation inhibition rate was increased,and the apoptosis rate of LNCaP cells was increased(all P＜0.05),while the expression of p38 MAPK protein showed no significant difference(P＞0.05).Compared with low-dose group,the expression of PSMA was decreased in medium-dose group,the expressions of p-p38 MAPK,Bax,Caspase-3,and P53 proteins were increased,the expression of Bcl-2 protein was decreased,the proliferation inhibition rate was increased,and the apoptosis rate of LNCaP cells was increased(all P＜0.05),while the expression of p38 MAPK protein showed no significant difference(P＞0.05).Compared with medium-dose group,the expression of PSMA was decreased in high-dose group,the expressions of p-p38 MAPK,Bax,Caspase-3,and P53 proteins were increased,the expression of Bcl-2 protein was decreased,the proliferation inhibition rate was increased,and the apoptosis rate of LNCaP cells was increased(all P＜0.05),while the expression of p38 MAPK protein showed no significant difference(P＞0.05).Conclusion Fuzheng Lishi Kang'ai decoction may suppress the proliferation and promote the apoptosis of LNCaP cells by inhibiting the expression of PSMA and activating the p38 MAPK signaling pathway.关键词
前列腺癌/扶正利湿抗癌方/PSMA/p38 MAPK/增殖/凋亡Key words
prostate cancer/Fuzheng Lishi Kang'ai decociton/PSMA/p38 MAPK/proliferation/apoptosis分类
医药卫生引用本文复制引用
梁文晋,王鹏,赵文兵..扶正利湿抗癌方对LNCaP细胞中PSMA表达及p38 MAPK通路的影响[J].山西医科大学学报,2023,54(11):1449-1454,6.基金项目
山西省自然科学基金资助项目(201901D111401) （201901D111401）
经方扶阳山西省重点实验室(202104010910011) （202104010910011）
