山西医科大学学报2023，Vol.54Issue(11)：1483-1487,5.DOI:10.13753/j.issn.1007-6611.2023.11.008
载淫羊藿苷和盐酸米诺环素明胶微球/骨水泥的构建及其性能研究
Construction and properties of calcium phosphate cement with icariin and minocycline hydrochloride-loaded gelatin microspheres
摘要
Abstract
Objective To construct calcium phosphate cements(CPCs)with icariin(ICA)and minocycline hydrochloride(MH)loaded gelatin microspheres(GMs)and study their properties for providing a bone substitute material in the treatment of peri-implantitis.Methods GMs,GMs loaded with ICA,GMs loaded with MH and GMs loaded with both drugs were prepared.The CPC/GMs(ICA+MH)was constructed by combining GMs(ICA+MH)with CPC,while the CPC/GMs(ICA)+GMs(MH)was constructed by combi-ning the mixture of GMs(ICA)and GMs(MH)in a mass ratio of 1:1 with CPC.The surface morphology of the materials was observed by scanning electron microscopy(SEM),and the phase was analyzed by X-ray diffraction.The effects of the material extracts on the proliferation of BMSCs were detected by CCK-8 assay,the effects of the material extracts and the mineralizing solution containing the extracts on the mineralization of BMSCs were detected by Alizarin red staining and quantitative analysis of calcium content.Results SEM showed that GMs were randomly dispersed and enfolded with CPC in CPC/GMs,CPC/GMs(ICA+MH),and CPC/GMs(ICA)+GMs(MH),and X-ray diffraction analysis showed that no new substance was formed after the combination of all kinds of GMs and CPC.The results of CCK-8 assay showed that the proliferation of BMSCs was significantly increased in CPC/GMs(ICA)+GMs(MH)group after cultured with the material extracts for 7 d,and it was higher than that in CPC/GMs group and CPC/GMs(ICA+MH)group(P＜0.05).After BMSCs were cultured in material extracts and mineralizing solutions containing the extracts for 21 d,the mineraliza-tion of BMSCs was statistically higher in CPC/GMs(ICA)+GMs(MH)group than that in CPC/GMs group and CPC/GMs(ICA+MH)group(P＜0.05).Conclusion CPC/GMs(ICA+MH)and CPC/GMs(ICA)+GMs(MH)can promote the osteogenic differentiation of BMSCs.CPC/GMs(ICA)+GMs(MH)has a stronger effect,and can be a preferred bone substitute material.关键词
淫羊藿苷/盐酸米诺环素/明胶微球/骨水泥/种植体周围炎Key words
icariin/minocycline hydrochloride/gelatin microspheres/calcium phosphate cements/peri-implantitis分类
医药卫生引用本文复制引用
刘宁,程楚童,刘天予,范晓龙,黄硕..载淫羊藿苷和盐酸米诺环素明胶微球/骨水泥的构建及其性能研究[J].山西医科大学学报,2023,54(11):1483-1487,5.基金项目
陕西省教育厅专项科研计划项目(20JK0889) （20JK0889）
西安市未央区科技计划项目(202331) （202331）
国家级大学生创新创业训练计划项目(202211840025) （202211840025）
国家自然科学基金资助项目(81801034) （81801034）
