实用医学杂志2023，Vol.39Issue(24)：3169-3174,6.DOI:10.3969/j.issn.1006-5725.2023.24.004
基于转录组测序探索腺苷脱氨酶1对宫颈癌细胞的调控机制
Screening of gene differential expression of adenosine deaminase RNA specific 1 in cervical cancer cells based on transcriptome sequencing technology
摘要
Abstract
Objective To analyze the regulation of gene expression by adenosine deaminase RNA specific 1(ADAR1)in cervical cancer cell line Hela using RNA-seq technology and provide theoretical basis for understanding the role of ADAR1 in the occurrence and progression of cervical cancer.Methods RNA-seq sequencing of normal and ADAR1 knockdown Hela cell lines to identify differentially expressed genes.By conducting enrichment analysis using KEGG Pathway,GO cellular,and GSEA,the study analyzes the relevant signaling pathways and biological processes involving ADAR1 in Hela cell lines.Results Differentially expressed genes are mainly enriched in immune and inflammation-related signaling pathways(such as TNF-α/NF-κB,NIK/NF-κB,Jak/Stat-IL-6),Hippo signaling pathway,TGF-β signaling pathway,and are involved in interferon response,cellular amino acid metabo-lism regulation,protein ubiquitination/deubiquitination,viral transcription,and other biological processes.Further analysis of the NF-κB signaling pathway revealed a significant increase in the mRNA expression levels of NF-κB1 and TRAF5 after ADAR1 knockdown.Conclusion ADAR1 may regulate the expression of NF-κB signaling pathway-related factors and thereby regulate the occurrence and development of cervical cancer.关键词
腺苷脱氨酶1/宫颈癌/转录组测序技术/NF-κB信号通路Key words
adenosine deaminase RNA specific 1/cervical cancer/RNA-seq technology/NF-κB signaling pathway分类
医药卫生引用本文复制引用
张华明,何婉珊,韩芸,陈冠桥,陈斌,韦之富,伍恒英,文斌..基于转录组测序探索腺苷脱氨酶1对宫颈癌细胞的调控机制[J].实用医学杂志,2023,39(24):3169-3174,6.基金项目
广东省中医药局科研项目(编号:20223033) （编号:20223033）
广州市科技计划项目(编号:202002030174) （编号:202002030174）
