实用妇产科杂志2023，Vol.39Issue(12)：929-933,5.
PDLIM3在子宫内膜异位组织中的表达及临床意义
The Expression of PDLIM3 in Endometriosis and its Clinical Significance
徐亦聪 1廉鑫 2杨雨青 1周圣涛1
作者信息
- 1. 四川大学华西第二医院妇产科,四川 成都 610041
- 2. 南京大学医学院附属鼓楼医院妇产科,江苏 南京 210093
- 折叠
摘要
Abstract
Objective:To investigate the expression of PDLIM3 in the ectopic and orthotopic endometrium of endometriosis(EMT)patients,as well as in healthy endometrium,and to explore its relationship with clinical signifi-cance.Methods:Bioinformatics analysis based on the Gene Expression Omnibus(GEO)database was conduc-ted,and a prospective cohort study was undertaken,involving 58 EMT patients treated at West China Second Hospital of Sichuan University and 27 patients with other benign gynecological diseases as the control group from December 2019 to July 2020.Ectopic and orthotopic endometrium samples were obtained from EMT patients,and normal control samples were obtained from patients with other benign gynecological diseases.Real-time quantita-tive polymerase chain reaction(qPCR),western blot,and immunohistochemistry were utilized to investigate PD-LIM3 expression in various tissue samples.Clinical parameters were analyzed to determine the correlation be-tween PDLIM3 and clinical features.Results:Compared to orthotopic endometrial tissues of EMT patients and normal endometrial tissues of control subjects,the mRNA(F =46.05,P<0.05)and protein(F =238.6,P<0.05)expression levels of PDLIM3 in ectopic endometrial lesions were significantly upregulated.The average optical density(AOD)of PDLIM3 protein obtained through immunohistochemical experiments in ectopic endometrial le-sions showed a positive correlation with the degree of dysmenorrhea(r =0.86,P<0.05)and CA 125 level(r = 0.41,P<0.05)in EMT patients.But no obvious correlation was found between the expression level of PDLIM3 and disease stage,menstrual volume,and CA199 level(P>0.05).Conclusions:The expression level of PDLIM3 in endometriosis lesions was significantly upregulated and correlated with the degree of dysmenorrhea and serum CA125 level.These findings suggest that PDLIM3 may play a significant role in the development and progression of endometriosis and could serve as a potential therapeutic target.关键词
子宫内膜异位症/PDZ和LIM结构域蛋白3/痛经Key words
Endometriosis/PDLIM3/Dysmenorrhea分类
医药卫生引用本文复制引用
徐亦聪,廉鑫,杨雨青,周圣涛..PDLIM3在子宫内膜异位组织中的表达及临床意义[J].实用妇产科杂志,2023,39(12):929-933,5.
