实用医学杂志2023，Vol.39Issue(24)：3195-3199,5.DOI:10.3969/j.issn.1006-5725.2023.24.008
骨髓增生异常综合征患者骨髓γδ T细胞及其亚群的水平及意义
Levels and significance of γδ T cells and their subpopulations in the bone marrow of MDS patients
摘要
Abstract
Objective To investigate the levels of γδ T cells and their subpopulations in bone marrow(BM)of patients with myelodysplastic syndrome(MDS),it aims to explore the immune deficiency status of BM microenvi-ronment in MDS patients.Methods BM samples were collected from MDS patients before and after treatment,as well as from normal donors.Multicolor flow cytometry was utilized to detect bone marrow γδ T cells and subpopulation levels.The changes of the T cell subsets after treatment were also analyzed.Results The levels of BM γδ T cells and follicular helper γδ T cells from MDS patients were significantly lower than those of normal donors(P<0.05).Among γδ T cells at different stages of differentiation,only the frequencies of naïve γδ T cells from MDS patients decreased significantly(P = 0.037),and there was no significant difference observed about central memory,effector memory,and terminally differentiated γδ T cells in MDS patients compared to normal donors(P>0.05).Although there was a slight decrease in PD1+γδ T cells and an increase in TIM3+γδ T cells,these differences were not statistically significant(P>0.05).In patients who achieved a curative effect,the proportions of γδ T cells and naive γδ T cells increased significantly after treatment,and the effector memory γδ T cells decreased significantly after treatment(P<0.05).After treatment,85.71%(6/7)of MDS patients showed a decrease in γδ+TIM3+ T cell levels to varying degrees.Conclusions The levels of γδ T cells and their subpopulations in the BM microenvironment of patients with MDS exhibit varying degrees of abnormalities.However,in patients who receive effective treatment,these abnormal γδ T cells can recover.By detecting the levels of γδ T cells and subpopulations,we can gain insights into the immune deficiency status of MDS.This information might serve as an indicator to assess treatment efficacy and provide valuable insights for anti-tumor immunotherapy.关键词
骨髓增生异常综合征/γδ T细胞/滤泡辅助性γδ T细胞/调节性γδ T细胞/程序性死亡受体1/T细胞免疫球蛋白黏蛋白分子3Key words
myelodysplastic syndrome/γδ T cells/follicular helper γδ T cells/regulatory γδ T cells/programmed death 1/T cell immunoglobulin and mucin-containing molecule 3分类
医药卫生引用本文复制引用
奚睿汀,耿素霞,黄欣,李敏明,邓程新,王玉连,黄励思,翁建宇,杜欣..骨髓增生异常综合征患者骨髓γδ T细胞及其亚群的水平及意义[J].实用医学杂志,2023,39(24):3195-3199,5.基金项目
国家自然科学基金项目(编号:82070128) （编号:82070128）
广东省自然科学基金项目(编号:2021A1515011436,2023A1515010170) （编号:2021A1515011436,2023A1515010170）
