生态环境学报2023，Vol.32Issue(11)：1942-1951,10.DOI:10.16258/j.cnki.1674-5906.2023.11.005
混合型生物炭对寒冷地区PAHs污染土壤微生物修复的强化作用
Strengthening Effect of Mixed Biochar on Microbial Remediation of PAHs Contaminated Soil in Cold Areas
摘要
Abstract
There are large areas in the Northern cold region that consist of contaminated soils with low and medium concentrations of polycyclic aromatic hydrocarbons(PAHs).These contaminants pose potential threats to both ecological safety and human health.In this paper,a mixed biochar(C500+W500)was used as a carrier,and Pseudomonas sp.S4 and Mortierella alpine J7 were used as mixed bacteria capable of degrading PAHs.The bioremediation materials were prepared using an adsorption immobilization method.The study aimed to investigate the degradation capabilities of mixed biochar and single-type biochar loaded with cold-tolerant mixed bacteria on soil compounds such as phenanthrene(Phe)and pyrene(Pyr)under low-temperature conditions,and the mechanisms and advantages of biochar in the process of microbial degradation of PAHs in soil were discussed.The results showed that the remediation effects of mixed biochar immobilized microorganisms on Phe and Pyr in soil were higher than those of free bacteria as well as single biochar immobilized bacteria.The remediation effect was related to the mixing ratio of biochar.After 30 days of remediation at 15℃,the removal rates of Phe and Pyr were 51.87%and 45.28%respectively,when using immobilized mixed biochar with 1.34%C500 and 0.67%W500 mixed biochar as the carrier(CWJ 2:1).These rates were higher than the removal rates achieved by free bacteria(25.81%and 23.65%)and single biochar immobilized group(15.63%‒18.96%and 13.62%‒16.07%).Furthermore,mixed biochar affected the migration of PAHs in soil.The addition of biochar to soil reduced the adsorption of Phe,facilitating the entry of PAHs into the bio-facies in soil and improving the microbial availability of PAHs.In terms of Phe's competitive adsorption,the equilibrium adsorption amounts of CK biochar,CK soil,CWsoil and CW were 0.01 μg·mg-1,25.45 μg·g-1,23.46 μg·g-1,and 26.46 μg·mg-1,respectively.The competitive adsorption process of soil Phe by biochar was found to align closely with the quasi second-order kinetic model.This study provides a theoretical reference for in-situ microbial remediation of PAH-contaminated soil in Northern cold areas.关键词
混合型生物炭/PAHs/固定化微生物/土壤修复/有机污染物/强化机理Key words
mixed-biochar/PAHs/immobilized microorganism/soil remediation/organic pollutants/strengthening mechanism分类
资源环境引用本文复制引用
苏丹,罗桥冰,董昱杉,杨彩霞,王鑫..混合型生物炭对寒冷地区PAHs污染土壤微生物修复的强化作用[J].生态环境学报,2023,32(11):1942-1951,10.基金项目
国家自然科学基金项目(52170163) （52170163）
辽宁省教育厅面上项目(LJKZ0092) （LJKZ0092）
沈阳市中青年科技创新人才支持计划(RC220064) （RC220064）
沈阳市科技计划项目(22-322-3-14) （22-322-3-14）
