山西医科大学学报2023，Vol.54Issue(12)：1585-1590,6.DOI:10.13753/j.issn.1007-6611.2023.12.006
盐酸小檗碱对肝纤维化大鼠肠道黏膜机械屏障的影响
Effect of berberine hydrochloride on mechanical barrier of intestinal mucosa in rats with liver fibrosis
摘要
Abstract
Objective To explore the effect and its mechanism of berberine hydrochloride on the mechanical barrier of intestinal mucosa in rats with liver fibrosis.Methods Sixty male SD rats were randomly divided into normal control group(n=20),model group(n-20)and berberine hydrochloride group(n=20).The liver fibrosis model was established by subcutaneous injection of 40％CC14 in olive oil solution in model group and berberine hydrochloride group.The rats in berberine hydrochloride group were treated with berberine hydrochloride 150 mg/(kg·d)by gavage at week 4 after model establishment.Inflammatory activity and fibrosis degree of liver tissue were scored by HE and Masson staining in each group.The structure and the arrangement of mucosal epithelial villi were observed by HE staining in each group.The serum contents of alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(TBIL)and albumin(ALB)were measured by automatic biochemical assay in each group.Blood diamine oxidase(DAO)activity was measured by spectrophotometry in each group.Serum tumor necrosis factor α(TNF-α)was determined by enzyme-linked immunoassay in each group.Immunohistochemistry and real-time PCR were used to detect the protein and mRNA levels of Occludin in small intestinal mucosa of rats in each group.Results G score and S score of liver tissue in model group and berberine hydrochloride group were significantly increased compared with normal control group(P＜0.05).G score and S score of liver tissue in berberine hydrochloride group were significantly lower than those in model group(P＜0.05).The intestinal mucosal structure was not complete and the intestinal villi were not neatly arranged in model group and berberine hydrochloride group.The structure of the small intestinal mucosa was intact and the intestinal villi were arranged neatly in berberine hydrochloride group.The serum levels of ALT,AST,TBIL,DAO and TNF-α in model group and berberine hydrochloride group were higher than those in normal control group(P＜0.05),and the serum levels of ALT,TBIL,DAO and TNF-α in berberine hydrochloride group were lower than those in model group(P＜0.05).The serum ALB level and the expressions of Occludin protein and mRNA in the small intestine were lower in model group and berberine hydrochloride group than those in normal control group(P＜0.05),and the levels of the above indexes in berberine hydrochloride group were higher than those in model group(P＜0.05).Conclusion The intestinal mucosa mechanical barrier is damaged during the liver fibrosis.Berberine hydrochloride can alleviate the damage of intestinal mucosa mechanical barrier during the liver fibrosis,which may be related to the reduction of TNF-α level.关键词
盐酸小檗碱/肝纤维化/肠黏膜/机械屏障/Occludin蛋白Key words
berberine hydrochloride/liver fibrosis/intestinal mucosa/mechanical barrier/Occludin protein分类
医药卫生引用本文复制引用
张建,侯洪涛,刘洪娜,崔子瑾,史林平,郑吉敏,王玉珍..盐酸小檗碱对肝纤维化大鼠肠道黏膜机械屏障的影响[J].山西医科大学学报,2023,54(12):1585-1590,6.基金项目
河北省卫健委医学科学研究重点课题项目(20180025 （）
20241262) （）
河北省中医药管理局科研计划项目(2019137) （2019137）
