陕西医学杂志2024，Vol.53Issue(1)：3-7,18,6.DOI:10.3969/j.issn.1000-7377.2024.01.001
山奈酚对缺氧/复氧损伤诱导心肌细胞凋亡的影响及机制研究
Effect and mechanism of kaempferol on cardiomyocytes apoptosis induced by hypoxia/reoxygenation injury
摘要
Abstract
Objective:To examine the effect and mechanism of kaempferol on myocardial cell apoptosis induced by hypoxia/reoxygenation injury.Methods:Rat cardiomyocytes H9C2 were randomly divided into control group,hy-poxia/reoxygenation group and kaempferol group.Cells in the control group were cultured in conventional incubator,and cells in the hypoxia/reoxygenation group were cultured in hypoxia incubator and conventional incubator alterna-tively.Cells in the kaempferol group were treated with 30 μmol/L kaempferol after incubation in the hypoxia/reoxy-genation.The flow cytometry and polynucleotide chain break detection technology were used to detect apoptosis lev-els in each group.Western blotting was used to detect the levels of apoptosis related proteins and calcium channel protein.Immunofluorescence technology was used to detect calcium ion concentration in cells.Results:Compared with the control group,the hypoxia/reoxygenation group showed an increase in apoptosis,the expression of calcium chan-nel protein Cav1.2 and calcium ion concentration in cells(all P＜0.05).Compared with the hypoxia/reoxygenation group,the kaempferol group showed a decrease in cell apoptosis,the expression of calcium channel protein Cav1.2 and calcium ion concentration in cells(all P＜0.05).Conclusion:kaempferol may inhibit rat cardiomyocytes H9C2 apoptosis induced by hypoxia/reoxygenation injury by reducing the expression of calcium channel protein Cav1.2 and calcium ion concentration in cells.关键词
山奈酚/大鼠心肌细胞H9C2/缺氧/复氧损伤/细胞凋亡/钙离子通道蛋白Cav1.2/细胞内钙离子浓度Key words
Kaempferol/Rat cardiomyocytes H9C2/Hypoxia/reoxygenation injury/Cell apoptosis/Calcium ion channel protein Cav1.2/Calcium ion concentration in cells分类
医药卫生引用本文复制引用
武金盼,陈继军,袁青,赵新春,何佩娟..山奈酚对缺氧/复氧损伤诱导心肌细胞凋亡的影响及机制研究[J].陕西医学杂志,2024,53(1):3-7,18,6.基金项目
陕西省自然科学基金资助项目(2021JM-246) （2021JM-246）
