山奈酚对缺氧/复氧损伤诱导心肌细胞凋亡的影响及机制研究OACSTPCD

Objective:To examine the effect and mechanism of kaempferol on myocardial cell apoptosis induced by hypoxia/reoxygenation injury.Methods:Rat cardiomyocytes H9C2 were randomly divided into control group,hy-poxia/reoxygenation group and kaempferol group.Cells in the control group were cultured in conventional incubator,and cells in the hypoxia/reoxygenation group were cultured in hypoxia incubator and conventional incubator alterna-tively.Cells in the kaempferol group were treated with 30 μmol/L kaempferol after incubation in the hypoxia/reoxy-genation.The flow cytometry and polynucleotide chain break detection technology were used to detect apoptosis lev-els in each group.Western blotting was used to detect the levels of apoptosis related proteins and calcium channel protein.Immunofluorescence technology was used to detect calcium ion concentration in cells.Results:Compared with the control group,the hypoxia/reoxygenation group showed an increase in apoptosis,the expression of calcium chan-nel protein Cav1.2 and calcium ion concentration in cells(all P＜0.05).Compared with the hypoxia/reoxygenation group,the kaempferol group showed a decrease in cell apoptosis,the expression of calcium channel protein Cav1.2 and calcium ion concentration in cells(all P＜0.05).Conclusion:kaempferol may inhibit rat cardiomyocytes H9C2 apoptosis induced by hypoxia/reoxygenation injury by reducing the expression of calcium channel protein Cav1.2 and calcium ion concentration in cells.