山奈酚对缺氧/复氧损伤诱导心肌细胞凋亡的影响及机制研究OACSTPCD
Effect and mechanism of kaempferol on cardiomyocytes apoptosis induced by hypoxia/reoxygenation injury
目的:探究山奈酚对缺氧/复氧损伤诱导心肌细胞凋亡的影响和机制.方法:利用随机数字法将H9C2大鼠心肌细胞随机分为三组,对照组细胞在常规孵箱培养,缺氧/复氧组细胞利用缺氧孵箱和常规孵箱交替培养,山奈酚组细胞在缺氧/复氧培养的基础上应用30 μmol/L山奈酚.分别利用流式细胞仪和多核苷酸链断裂技术检测各组细胞凋亡水平,利用蛋白印迹技术检测凋亡相关蛋白和钙离子通道蛋白含量,利用免疫荧光技术检测细胞中钙离子浓度.结果:和对照组比较,缺氧/复氧组细胞凋亡…查看全部>>
Objective:To examine the effect and mechanism of kaempferol on myocardial cell apoptosis induced by hypoxia/reoxygenation injury.Methods:Rat cardiomyocytes H9C2 were randomly divided into control group,hy-poxia/reoxygenation group and kaempferol group.Cells in the control group were cultured in conventional incubator,and cells in the hypoxia/reoxygenation group were cultured in hypoxia incubator and conventional incubator alterna-tively.Cells in the kaempfer…查看全部>>
武金盼;陈继军;袁青;赵新春;何佩娟
空军军医大学西京医院,陕西西安 710032空军军医大学西京医院,陕西西安 710032空军军医大学西京医院,陕西西安 710032空军军医大学西京医院,陕西西安 710032空军军医大学西京医院,陕西西安 710032
临床医学
山奈酚大鼠心肌细胞H9C2缺氧/复氧损伤细胞凋亡钙离子通道蛋白Cav1.2细胞内钙离子浓度
KaempferolRat cardiomyocytes H9C2Hypoxia/reoxygenation injuryCell apoptosisCalcium ion channel protein Cav1.2Calcium ion concentration in cells
《陕西医学杂志》 2024 (1)
3-7,18,6
陕西省自然科学基金资助项目(2021JM-246)
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