细颗粒物致人肺泡上皮细胞炎性反应关键组分及机制探究OACSTPCD

Objective:To observe the effect and mechanism of different components of fine particulate matter(PM2.5)on the proliferation of human type Ⅱ alveolar epithelial cells(A549).Methods:The whole particles,liposolu-ble-soluble and water-soluble of PM2.5 were prepared,and the effects of different components of PM2.5 on the mor-phology of A549 were observed.MTT assay was used to evaluate the effect of different components of PM2.5 on cell proliferation.ELISA was used to detect the effect of different components of PM2.5 on the expression of inflammatory factors.On the basis of PM25 component exposure,MAPK signaling pathway inhibitor and dexamethasone were giv-en to A549,and the changes in proliferation,inflammatory factors and the expression of MAPK signaling pathway re-lated proteins were detected.Results:The whole particles and liposoluble-soluble of PM2 5(WPPM2.5 and LSPM2 5)can inhibit the proliferation of A549 cells in a time-and concentration-dependent manner and the IC50 were 199.9,142.1 μg/ml,respectively.The inflammatory factors in cell culture supernatants increased significantly after WPPM2.5 and LSPM2.5 exposure.MAPK signaling pathway inhibitors(U0126,SB203580,SP600125)and dexametha-sone significantly enhanced the inhibitory effect of WPPM2.5 and LSPM2.5 on A549 cells,inhibited the increase in the expression of inflammatory factors induced by WPPM2 5 and LSPM2.5,and promoted the phosphorylation of JNK and ERK1/2.Conclusion:The effect of PM2.5on A549 cell viability and inflammation was related with water-insoluble components,and the mechanism may be related to the activation of ERK1/2 MAPK and JNK MAPK signaling path-ways.