陕西医学杂志2024，Vol.53Issue(1)：8-12,5.DOI:10.3969/j.issn.1000-7377.2024.01.002
细颗粒物致人肺泡上皮细胞炎性反应关键组分及机制探究
Exploration of critical components for PM2.5 induced inflammatory reaction in human alveolar epithelial cells
摘要
Abstract
Objective:To observe the effect and mechanism of different components of fine particulate matter(PM2.5)on the proliferation of human type Ⅱ alveolar epithelial cells(A549).Methods:The whole particles,liposolu-ble-soluble and water-soluble of PM2.5 were prepared,and the effects of different components of PM2.5 on the mor-phology of A549 were observed.MTT assay was used to evaluate the effect of different components of PM2.5 on cell proliferation.ELISA was used to detect the effect of different components of PM2.5 on the expression of inflammatory factors.On the basis of PM25 component exposure,MAPK signaling pathway inhibitor and dexamethasone were giv-en to A549,and the changes in proliferation,inflammatory factors and the expression of MAPK signaling pathway re-lated proteins were detected.Results:The whole particles and liposoluble-soluble of PM2 5(WPPM2.5 and LSPM2 5)can inhibit the proliferation of A549 cells in a time-and concentration-dependent manner and the IC50 were 199.9,142.1 μg/ml,respectively.The inflammatory factors in cell culture supernatants increased significantly after WPPM2.5 and LSPM2.5 exposure.MAPK signaling pathway inhibitors(U0126,SB203580,SP600125)and dexametha-sone significantly enhanced the inhibitory effect of WPPM2.5 and LSPM2.5 on A549 cells,inhibited the increase in the expression of inflammatory factors induced by WPPM2 5 and LSPM2.5,and promoted the phosphorylation of JNK and ERK1/2.Conclusion:The effect of PM2.5on A549 cell viability and inflammation was related with water-insoluble components,and the mechanism may be related to the activation of ERK1/2 MAPK and JNK MAPK signaling path-ways.关键词
PM2.5/A549细胞/细胞毒性/炎症反应/丝裂原活化蛋白激酶信号通路/关键组分Key words
PM2.5/Critical components/A549 cells/Cytotoxicity/Inflammatory response/MAPK signaling pathway分类
医药卫生引用本文复制引用
王荣,张邱栋,张懿烨,贺红梅,王娜娜,秦蓓..细颗粒物致人肺泡上皮细胞炎性反应关键组分及机制探究[J].陕西医学杂志,2024,53(1):8-12,5.基金项目
国家自然科学基金资助项目(81903288) （81903288）
西安医学院校级科技创新团队建设项目(2021TD03) （2021TD03）
国家级、省级、校级大学生创新创业训练计划项目(202311840020,121522107,121523020) （202311840020,121522107,121523020）
西安医学院科研能力提升计划项目(2022NLTS070) （2022NLTS070）
