川芎嗪调控Wnt/β-连环蛋白信号通路对冠心病大鼠心肌细胞凋亡的机制研究OACSTPCD

Objective:To investigate the effect of tetramethylpyrazine regulation of Wnt/β-catenin signaling pathway on cardiomyocyte apoptosis in rats with coronary heart disease(CHD).Methods:A total of 50 adult male SD rats were selected and divided into control group,model group,tetramethylpyrazine low dose group,medium dose group and high dose group with 10 rats each.The CHD rat model was established by high fat feed and fat emulsion intragastric administration.The rats in the control group and model group were injected with equal volume 0.9％so-dium chloride solution through tail vein,and tetramethylpyrazine was injected intraperitoneally at low,medium and high doses.All groups were treated continuously for 3 weeks.After intervention,the cardiac function and serum lac-tate dehydrogenase(LDH),creatine kinase(CK)and CK isoenzyme(CK-MB)levels of rats in each group were de-tected.Myocardial tissue structure was observed by HE staining,and myocardial apoptosis was detected by TUNEL.The mRNA and protein expressions of Wnt3a and β-catenin were detected by qRT-PCR and Western blot.Results:The heart function of the model group was lower than that of the control group and the low dose,medium dose and high dose groups(all P＜0.05).The serum levels of LDH,CK and CK-MB in model group were higher than those in control group and low,medium and high dose groups(all P＜0.05).The myocardial cells of the control group were equal in size,and the myocardial fibers were not swollen and tightly arranged;the myocardial fibers of the model group showed obvious swelling and breakage,and the cell arrangement was irregular,with a large number of inflam-matory cells infiltrating;the myocardial damage of the tetramethylpyrazine low,medium and high dose groups was gradually improved,and the cell edema degeneration was reduced,with only a small number of inflammatory cells infiltrating.The mRNA and protein expressions of Wnt3a and β-catenin in myocardium of tetramethylpyrazine high dose group were lower than those of low and medium dose groups(all P＜0.05).Conclusion:Ligustrazine can improve apoptosis and reduce cell damage of rat cardio-myocytes,and its mechanism may be related to the regulation of Wnt/β-catenin signaling pathway related protein expression.