天津中医药2024，Vol.41Issue(1)：87-92,6.DOI:10.11656/j.issn.1672-1519.2024.01.17
基于JAK2/STAT3信号通路探讨升清降浊制动颗粒对多发性抽动症模型大鼠的作用及机制
Exploring the effect and mechanism of Shengqing Jiangzhuo Zhidong Granule on Tourette's syndrome model rats based on JAK2/STAT3 signal pathway
摘要
Abstract
[Objective]To explore the effect and mechanism of Shengqing Jiangzhuo Zhidong Granule on Tourette's syndrome(TS)model rats based on Janus kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3)signal pathway.[Methods]SD rats were grouped into control group,TS group,low dose Shengqing Jiangzhuo Zhidong Granule group(0.645 mg/kg),high dose Shengqing Jiangzhuo Zhidong Granule group(2.58 mg/kg),haloperidol group(200 μg/kg),Coumermycin A1(JAK2 pathway activator)group(4 mg/kg),and high dose Shengqing Jiangzhuo Zhidong Granule+Coumermycin A1 group(2.58 mg/kg+4 mg/kg),12 in each group.Except for the control group,rats in other groups were all constructed with TS model.After successful modeling,the drug was administered once a day for 21 days.The changes in motor behavior score and stereotyped behavior score of rats were detected;the levels of serum interleukin(IL)-1β,tumor necrosis factor-α(TNF-α),IL-6,and the contents of dopamine(DA),dopamine transporter(DAT)and dopamine D2 receptor(DRD2)in striatum were detected by enzyme-linked immunosorbent assay(ELISA);DNA notch end labeling(TUNEL)staining was applied to detect neuronal apoptosis in rat striatum;Western blot was applied to detect the expression of p-JAK2 and p-STAT3 proteins in rat striatum.[Results]Compared with the control group,the motor behavior score,stereotyped behavior score,IL-1β,TNF-α,IL-6 levels,DRD2 content,neuron apoptosis rate,p-JAK2,p-STAT3 protein expression in TS group were higher,and the DA and DAT contents were lower(P＜0.05);compared with TS group,the motor behavior score,stereotyped behavior score,IL-1β,TNF-α,IL-6 levels,DRD2 content,neuron apoptosis rate,p-JAK2,p-STAT3 protein expression of rats in the low dose Shengqing Jiangzhuo Zhidong Granule group,high dose Shengqing Jiangzhuo Zhidong Granule group and haloperidol group were lower,and the DA and DAT contents were higher(P＜0.05);compared with TS group,the scores of motor behavior,stereotyped behavior,IL-1β,TNF-α,IL-6 levels,DRD2 content,neuron apoptosis rate,p-JAK2,p-STAT3 protein expression of rats in Coumermycin A1 group were higher,and the DA and DAT contents were lower(P＜0.05);coumermycin A1 attenuated the protective effect of high-dose Shengqing Jiangzhuo Zhidong Granules on TS model rats.[Conclusion]Shengqing Jiangzhuo Zhidong Granules can inhibit neuroinflammation,neuron apoptosis and promote DA balance in TS rats,which may be related to the inhibition of JAK2/STAT3 signaling pathway.关键词
升清降浊制动颗粒/Janus激酶2/信号转导和转录激活子3信号通路/多发性抽动症/多巴胺Key words
Shengqing Jiangzhuo Zhidong Granule/JAK2/STAT3 signal pathway/Tourette's syndrome/dopamine分类
医药卫生引用本文复制引用
王素亭,彭琰琰,韩雪,任燕,葛国岚,潘丹萍,高国财..基于JAK2/STAT3信号通路探讨升清降浊制动颗粒对多发性抽动症模型大鼠的作用及机制[J].天津中医药,2024,41(1):87-92,6.基金项目
河南省医学科技攻关计划联合共建立项项目(LHGJ2020066). （LHGJ2020066）
