陕西医学杂志2024，Vol.53Issue(1)：28-31,36,5.DOI:10.3969/j.issn.1000-7377.2024.01.006
全反式维甲酸联合小剂量阿糖胞苷对急性髓系白血病细胞磷脂酰肌醇3激酶/蛋白质丝氨酸苏氨酸激酶信号通路的作用机制研究
Mechanism of all-trans retinoic acid combined with low-dose cytarabine on PI3K/AKT signaling pathway of acute myeloid leukemia cells
摘要
Abstract
Objective:To investigate the mechanism of all-trans retinoic acid(ATRA)combined with low-dose cytarabine(Ara-C)on the signaling pathway of phosphatidylinositol 3 kinase(PI3K)/protein serine threonine kinase(AKT)in acute myeloid leukemia cells.Methods:Human acute myeloid leukemia cells HL-60 were selected.They were divided into blank control group(HL-60 cells without any treatment),Ara-C group(adding 0.5 μmol/L Ara-C),ATRA group(adding 2 μmol/L ATRA),ATRA+Ara-C group(adding 2 μmol/L ATRA and 0.5 μmol/L Ara-C).Culture continued for 24,48 and 72 hours.CCK8 was used to detect the viability of HL-60 cells,AnnexinV double staining was used to detect the apoptosis of HL-60 cells,qRT-PCR was used to detect the mRNA expression of PI3K and AKT,and Western blot was used to detect the protein expression of PI3K/AKT signaling pathway.The cell morphology was observed.Results:The activity of HL-60 cells in blank control group was higher than that in Ara-C+ATRA group,Ara-C group and ATRA group,and the activity of HL-60 cells in Ara-C and ATRA groups was higher than that in Ara-C+ATRA group(all P＜0.05).The apoptosis rate of HL-60 cells in Ara-C+ATRA group was higher than that in Ara-C and ATRA groups,and the apoptosis rate of HL-60 cells in Ara-C and ATRA groups was higher than that in blank control group(all P＜0.05).The body of HL-60 cells was mostly round with nodular pro-trusion,and the nuclei were mostly round like.The chromatin in the Ara-C and ATRA groups was condensed,the color became darker,and some of the nuclei became smaller.The chromatin in the Ara-C+ATRA group was con-densed,the color became darker,and the nuclear pyknosis and nuclear fragmentation were observed.The expressions of P13K and AKT mRNA in HL-60 cells of blank control group were higher than those in Ara-C and ATRA groups,and the expressions of PI3K and AKT mRNA in HL-60 cells of Ara-C and ATRA groups were higher than those in Ara-C+ATRA group(all P＜0.05).The expressions of P-PI3K and P-AKT in HL-60 cells of blank control group were higher than those in Ara-C and ATRA groups,and the expressions of P-PI3K and P-AKT in HL-60 cells of Ara-C and ATRA groups were higher than those in Ara-C+ATRA group(all P＜0.05).Conclusion:Ara-C+ATRA can promote the apoptosis of HL-60 cells by inhibiting the activation of PI3K/AKT signaling pathway.关键词
全反式维甲酸/阿糖胞苷/急性髓系白血病/HL-60细胞/磷脂酰肌醇3激酶/蛋白质丝氨酸苏氨酸激酶信号通路/细胞凋亡Key words
All-trans retinoic acid/Cytarabine/Acute myeloid leukemia/HL-60 cells/PI3K/AKT signaling pathway/Apoptosis分类
医药卫生引用本文复制引用
杨白梅,鲁猛,骆思君,王志华,伍华英,王芳,赵耀顺..全反式维甲酸联合小剂量阿糖胞苷对急性髓系白血病细胞磷脂酰肌醇3激酶/蛋白质丝氨酸苏氨酸激酶信号通路的作用机制研究[J].陕西医学杂志,2024,53(1):28-31,36,5.基金项目
河北省医学科学研究计划课题(20201252) （20201252）
