陕西医学杂志2024，Vol.53Issue(1)：32-36,5.DOI:10.3969/j.issn.1000-7377.2024.01.007
持续高糖状态对Kv11.1离子通道蛋白表达的影响
Effect of continuous high glucose on the expression of Kv11.1 ion channel protein
摘要
Abstract
Objective:To investigate the effect of continuous high concentration glucose intervention on the ex-pression of Kv11.1 ion channel protein.Methods:①The HERG gene was inserted into the eukaryotic expression vec-tor pEGFP-N1 expressing green fluorescent protein by double enzyme digestion method and gene reconstruction tech-nology to construct the expression vector pEGFP-N1-HERG of Kv11.1 ion channel protein and sequence it for verifi-cation.②After the identification of pEGFP-N1-HERG expression vector was successful,HEK293T cells were trans-fected with liposomes,and the average expression of HERG ion channel protein green fluorescence was detected by flow cytometry after 48 hours of intervention with different glucose concentrations(5,17.5,30 mmol/L).Results:The average fluorescence intensity of pEGFP-N1-HERG fusion protein detected by flow cytometry was 218.87(5 mmol/L),174.83(17.5 mmol/L),142.90(30 mmol/L)respectively after continuous intervention with different concentrations of glucose.There was significant difference among the three groups(P＜0.05).Conclusion:Sustained hyperglycemia inhibits the expression of Kv11.1 ion channel protein,which provides theoretical basis and experimen-tal basis for QT interval extension in diabetes patients with long-term hyperglycemia.关键词
HERG基因/真核表达载体/高糖干预/Kv11.1/蛋白表达Key words
HERG gene/Eukaryotic expression vector/High sugar intervention/Kv11.1/Protein expression分类
医药卫生引用本文复制引用
韩稳琦,王毅,陈海潮,尤红俊,邓纪钊,祁杰..持续高糖状态对Kv11.1离子通道蛋白表达的影响[J].陕西医学杂志,2024,53(1):32-36,5.基金项目
陕西省科学技术研究发展计划资助项目(2022SF-152) （2022SF-152）
陕西省人民医院科技人才支持计划项目(2022JY-69) （2022JY-69）
陕西省人民医院科技发展孵化基金资助项目(2022YJY-53) （2022YJY-53）
