陕西医学杂志2024，Vol.53Issue(1)：37-41,5.DOI:10.3969/j.issn.1000-7377.2024.01.008
红景天苷对Lewis肺癌小鼠恶性胸腔积液的抑制作用及其对免疫功能的影响
Inhibitory effect of SALidroside on malignant pleural effusion and its effect on immune function in mice with Lewis lung cancer
摘要
Abstract
Objective:To investigate the inhibitory effect of salidroside(SAL)on the formation of malignant pleural effusion and its effect on immune function in mice with lung cancer.Methods:The malignant pleural effusion(MPE)model of C57BL/6 mice was established by thoracic injection of Lewis lung cancer cells(LLC).After model-ing,they were randomly divided into normal group,model group,SAL group,cisplatin group(DDP group)and sali-droside+cisplatin combination group(SAL+DDP group).After the experiment,the pleural fluid of each group was collected and its volume and life cycle were measured.The levels of tumor necrosis factor(TNF-α),hypoxia inducible factor 1alpha(HIF-1α)and vascular endothelial growth factor(VEGF)in serum of MPE mice were detected by ELISA.T cell subsets CD3+,CD4+,CD8+,CD4+/CD8+and the percentage of NK cells were quantitatively detected by flow cytometry.Results:After treatment,compared with the model group,the survival period of the MPE model mice in the DDP group and the combination group was significantly prolonged(all P＜0.01);the MPE volumes of SAL group,DDP group and combination group were significantly reduced(all P＜0.01);the levels of TNF-α in pe-ripheral blood of SAL group,DDP group and combination group were significantly increased,and the levels of HIF-1αand VEGF were significantly decreased(all P＜0.05);CD3+in SAL group,DDP group and combination group were significantly increased(all P＜0.05);CD4+was significantly increased in SAL group and combination group(all P＜0.05),but not in DDP group(P＞0.05);CD8+,CD4+/CD8+and NK were significantly increased in SAL group and combination group(all P＜0.05),but the decrease in DDP group was not statistically significant(P＞0.05).Conclusion:SAL combined with cisplatin perfusion can significantly inhibit MPE production in lung cancer mice,which may be related to inhibition of HIF-1α-VEGF molecular pathway and activation of immune function.关键词
红景天苷/胸腔积液/肺癌/顺铂/血管内皮生长因子/肿瘤坏死因子-αKey words
Salidroside/Pleural effusion/Lung cancer/Cisplatin/VEGF/TNF-α分类
医药卫生引用本文复制引用
王松海,凡瑾,陈捷..红景天苷对Lewis肺癌小鼠恶性胸腔积液的抑制作用及其对免疫功能的影响[J].陕西医学杂志,2024,53(1):37-41,5.基金项目
陕西省自然科学基础研究项目(2020JM-670) （2020JM-670）
