现代医药卫生2023，Vol.39Issue(24)：4145-4147,4153,4.DOI:10.3969/j.issn.1009-5519.2023.24.002
重庆市中心城区汇集浓缩血小板制备关键控制点确认探讨
Discussion on the confirmation of critical control points for the preparation of pooled concentrated plateletsin the central city of Chongqing
摘要
Abstract
Objective To discuss the confirmation of critical control points and parameters in the prepa-ration of pooledconcentrated platelets,so as to improve the product quality of pooled platelets and optimize the preparation process.Methods A total of 84 street blood samples(400 mL each)were randomly selected.The buffy coat(BC)method was adopted,and BC was preserved by standing overnight.According to the whole blood platelet content and BC recovery rate,the BC fraction of pooled concentrated platelets was determined.According to the centrifuge performance and centrifugal effect,the appropriate centrifugal parameters was confirmed.The related quality indexes of pooled concentrated platelets and mechanically collected platelets,such as volume,platelet content,red blood cell mixing amount,white blood cell count,pH value at the end of storage and aseptic experiment,were compared.Results A total of six BC prepared from fresh 400mL whole blood were mixed to prepare pooled concentrated platelets.The first whole blood centrifugal parameters and the second BC centrifugal parameters of the centrifuge(Thermo cryofuge 8,USA)were:centrifugal force 2 283 g and 291 respectively;The acceleration was all 9 g;The deceleration was all 4 g;The time was all 12 min,and the parameters of the third centrifugation(reducing red blood cells)were centrifugal force of 145 g,accelera-tion of 9 g,deceleration of 4 g and time of 5 min,and the centrifugation effect was well confirmed.Comparing the test results of 14 platelets collected separately at the first and fifth days,the volume and content and the a-mount of red blood cells mixed in of pooled concentrated platelets were higher than those in the platelet col-lected by machine,with statistical significance(P＜0.05).There was no significant difference between white blood cell count and pH value at the end of storage(P＞0.05),and the results of aseptic test were all aseptic growth.All the quality indexes of pooled concentrated platelets were within the national standard except the volume.Conclusion Good control of critical control points can effectively improve the success rate of preparation.关键词
汇集血小板/白膜法/离心参数/关键控制点Key words
Pooledplatelet/Buffy coatmethod/Centrifugal parameters/Critical control point分类
医药卫生引用本文复制引用
周琼,杨冬燕,骆展鹏,卿芸,贺冬梅,林兰,曾北南..重庆市中心城区汇集浓缩血小板制备关键控制点确认探讨[J].现代医药卫生,2023,39(24):4145-4147,4153,4.基金项目
九龙坡区科技局技术预见与制度创新项目(2022-3-007-Z). （2022-3-007-Z）
