湖北科技学院学报（医学版）2023，Vol.37Issue(6)：465-469,5.DOI:10.16751/j.cnki.2095-4646.2023.06.0465
茯苓三萜对α-葡萄糖苷酶和α-淀粉酶的抑制作用及酶动力学研究
The Inhibition Effects and Enzyme Kinetics of Poria Cocos Triterpenes on α-glucosidase and α-amylase
摘要
Abstract
Objective To investigate the inhibition effects and kinetic properties of Poria cocos triterpe-nes on α-glucosidase and α-amylase.Methods The inhibitory activities of different concentrations of Poria co-cos triterpene on α-glucosidase and α-amylase were tested in vitro,and the kinetic properties were analyzed by Lineweaver-Burk.Results The in vitro enzyme activity inhibition assay showed that different concentrations of Poria triterpenoids inhibited both α-glucosidase and α-amylase in vitro,and their half-maximum inhibitory con-centrations(IC50)were 1.40μg/mL and 10.43μg/mL,respectively,and the inhibitory effect of Poria triterpe-noids was reversible and non-competitive inhibition on α-glucosidase,and the inhibitory effect on α-amylase was reversible mixed inhibition type.Conclusion The Poria cocos triterpenes have hypoglycemic activity,and show concentration-dependent manner.This study can provide a scientific basis for the research and development of Poria cocos hypoglycemic drugs.关键词
茯苓三萜/α-葡萄糖苷酶/α-淀粉酶/降血糖/酶动力学Key words
Poria cocos triterpenes/α-Glucosidase/α-Amylase/Blood glucose lowering/Enzyme kinet-ics分类
医药卫生引用本文复制引用
高满军,黄兴琼,莫启贵,陈迷,赵宝清,张丹丹..茯苓三萜对α-葡萄糖苷酶和α-淀粉酶的抑制作用及酶动力学研究[J].湖北科技学院学报（医学版）,2023,37(6):465-469,5.基金项目
糖尿病心脑血管病变湖北省重点实验室开放基金项目(2022TNB05) （2022TNB05）
湖北科技学院博士科研启动基金(BK202324) （BK202324）
