湖北科技学院学报（医学版）2023，Vol.37Issue(6)：470-474,5.DOI:10.16751/j.cnki.2095-4646.2023.06.0470
HPLC法考察不同影响因素下雪诺同黄体酮阴道缓释凝胶的体外药物释放度
HPLC Method Was Used to Investigate the Drug Release of Crinone® Progesterone Vaginal Sustained Release Gel in Vitro with Different Influencing Factors
宋歌 1梅兴国 2陈振雄3
作者信息
- 1. 湖北科技学院医学部药学院,湖北 咸宁 437100
- 2. 军事医学科学院毒物药物研究所
- 3. 咸宁市第一人民医院
- 折叠
摘要
Abstract
Objective To investigate the cumulative dissolution rate of Crinone® progesterone vaginal sustained release gel(8%)under different factors,and to screen out the best factors and levels.Methods Or-thogonal test was designed.Basket method,a determination method of drug release,was used to analyze the drug dissolution rate of progesterone vaginal sustained release gel under the same chromatographic conditions with different filter materials,pore sizes and release media.The rotation speed was 100 r/min and the water tempera-ture was(37±0.5)℃.Results The material of the filter membrane had a significant effect on the dissolution rate of the drug in the gel,and the range value(R)was 74.88.The pore size of the filter membrane and the dissolution medium had little influence on the dissolution rate,with R values of 8.43 and 8.78,respectively.The order of influencing factors was factor A(filter material)>Factor C(dissolution medium)>factor B(fil-ter aperture).Nylon membrane was more likely to have reverse osmosis than polycarbonate fiber membrane.Conclusion The best drug dissolution results were obtained when the filter membrane was 0.05μm polycarbon-ate fiber membrane and the medium was 0.5%sodium dodecyl sulfate phosphate buffer(pH 4.0).关键词
黄体酮/黄体酮阴道缓释凝胶/体外释放度/影响因素分析Key words
Progesterone/Progesterone vaginal sustained release gel/In vitro dissolution/Analysis of influencing factors分类
医药卫生引用本文复制引用
宋歌,梅兴国,陈振雄..HPLC法考察不同影响因素下雪诺同黄体酮阴道缓释凝胶的体外药物释放度[J].湖北科技学院学报（医学版）,2023,37(6):470-474,5.
