湖北科技学院学报（医学版）2023，Vol.37Issue(6)：491-494,4.DOI:10.16751/j.cnki.2095-4646.2023.06.0491
基于吲哚的溶酶体荧光探针的合成及应用
Synthesis and Application of Indole-Based Lysosome Fluorescent Probe
陈涛 1梁梓健 2桑亚男 1王自瑶 2柴方园 1晏可 1张璐 3高涛4
作者信息
- 1. 湖北科技学院医学部药学院,湖北 咸宁 437100||湖北科技学院非动力核技术化学与生物学院
- 2. 湖北科技学院医学部药学院,湖北 咸宁 437100
- 3. 湖北科技学院非动力核技术化学与生物学院
- 4. 湖北科技学院医学部药学院,湖北 咸宁 437100||湖北科技学院非动力核技术化学与生物学院||湖北科技学院辐射化学与功能材料湖北省重点实验室
- 折叠
摘要
Abstract
Objective To design and synthesize a fluorescent probe for lysosomal imaging.Methods The compound(E)-3-(2-pyridine-4-vinyl)-1H-indole(PVI)was synthesized by using racholine and 4-pyri-dine formaldehyde as substrates and n-tributylphosphine as catalyst.The structure of the compounds was charac-terized by nuclear magnetic resonance(NMR).The relationship between the probe and the pH of the solution was explored by fluorescence spectroscopy and UV-vis absorption spectroscopy.The mechanism of pH response was estimated by theoretical calculation.The cell imaging was observed by inverted fluorescence microscope.The lysosomal co-localization imaging was recorded by confocal laser scanning microscopy.Results The fluores-cence intensity of PVI was gradually increased with the decrease of pH when the pH range from 4.00 to 7.00.PVI had low cytotoxicity and could be used for imaging PC-3 prostate cancer cells and their lysosomes.Conclu-sion A pH-responsive fluorescent probe PVI has been synthesized,and shows the advantages of low cytotoxicity,good biocompatibility,and high fluorescence intensity.As a lysosome fluorescent probe with,it can be used for lysosome localization and imaging,and has commercial application value.关键词
荧光探针/pH响应/细胞成像/溶酶体Key words
Fluorescent probe/pH-Response/Imaging of cells/Lysosome分类
医药卫生引用本文复制引用
陈涛,梁梓健,桑亚男,王自瑶,柴方园,晏可,张璐,高涛..基于吲哚的溶酶体荧光探针的合成及应用[J].湖北科技学院学报（医学版）,2023,37(6):491-494,4.
