湖北科技学院学报（医学版）2023，Vol.37Issue(6)：499-505,7.DOI:10.16751/j.cnki.2095-4646.2023.06.00499
基于网络药理学和体外实验探析盐酸药根碱对人骨肉瘤的机制研究
Exploring the Mechanism of Jatrorrhizine Hydrochloride on Human Osteosarcoma Based on Network Pharmacology and in Vitro Experiments
摘要
Abstract
Objective To investigate the potential targets of jatrorrhizine hydrochloride(JH)on human osteosarcoma cells(HOS)and its effects on cell proliferation,migration,invasion and mechanism of action based on network pharmacology,molecular docking techniques and cellular assays.Methods Firstly,the con-stituent targets of JH and HOS-related disease targets were screened through the database,and as a way to con-struct and map the drug-target-disease visualization network and protein interaction network(PPI)and perform GO and KEGG enrichment analysis.And then the validation of potential targets of action(active ingredients with the highest number of targets and proteins with the highest degree of freedom in protein interactions)was performed by used molecular docking software.HOS cells were treated with different concentrations of JH,and the inhibitory effect of JH on the proliferation of HOS was confirmed by MTT assay,plate clone formation,and Transwell invasion and migration assay.Results In this study,a total of 26 anti-osteosarcoma targets of JH were obtained,and the enrichment analysis suggested that JH exerted its anti-osteosarcoma effects through various signaling pathways.The molecular docking showed good binding activity between JH and the main targets.Com-pared with the blank group,JH significantly reduced the activity,proliferation and migration ability of HOS.Conclusion JH might effectively inhibit HOS proliferation through multiple targets.In vitro cellular assays showed that JH could inhibite the proliferation,invasion and migration of HOS with the increasing of concentra-tions,and therefore might be as an effective potential anti-osteosarcoma drug.关键词
盐酸药根碱/人骨肉瘤/增殖/网络药理学/分子对接Key words
Jatrorrhizine hydrochloride/Human osteosarcoma/Proliferation/Network pharmacology/Molecular docking分类
药学引用本文复制引用
赵冰洁,阮英,宁志丰..基于网络药理学和体外实验探析盐酸药根碱对人骨肉瘤的机制研究[J].湖北科技学院学报（医学版）,2023,37(6):499-505,7.基金项目
湖北科技学院五官专项(202002) （202002）
湖北省重点研发计划大健康专项(2022BCE011) （2022BCE011）
