徐州工程学院学报（自然科学版）2023，Vol.38Issue(4)：5-13,9.
双水相法萃取灵芝醇提液中三萜类化合物的工艺优化
The Optimized Process for the Extraction of Triterpenoids from the Alcoholic Extract of Ganoderma lucidum with Aqueous Two-Phase System
摘要
Abstract
The method of extracting triterpenoids from alcohol extract of Ganoderma lucidum was studied by constructing the aqueous two-phase system of salt and alcohol.The extraction efficiency of Ganoderma lucidum triterpenes was investigated by an alcohol-salt system consisting of 13 inorganic salts and ethanol.It was found that the EtOH-NaH2 PO4 system had the highest extraction efficiency.The effects of NaH2 PO4 concentration in aqueous solution,mass fractions of ethanol and NaCl,and pH of aqueous phase on extraction efficiency were investigated by single factor experiment.The optimized process conditions were obtained by orthogonal experiment:the mass fractions of NaH2 PO4,water,ethanol and Ganoderma lucidum alcohol extract were 10%,53%,27%and 10%,respectively.Before extraction,solid NaOH was used to adjust the pH of aqueous phase to 5.At the same time,the results of the two methods of extraction,namely,the aqueous two-phase and the alkaline-acid solvent,were also compared.It was found that the extraction rate of triterpenes of Ganoderma lucidum by the aqueous two-phase method was 47.366%,which was higher than that by the alkaline extraction and acid solution(44.933%).Furthermore,the aqueous two-phase extraction was proved to be an efficient and green extraction method.关键词
灵芝三萜/双水相体系/萃取/正交试验/工艺优化Key words
Ganoderma triterpenoids/aqueous two-phase system/extraction/orthogonal experiment/process optimization分类
生物学引用本文复制引用
韩伟,姚泽远,卜原玲,王承潇..双水相法萃取灵芝醇提液中三萜类化合物的工艺优化[J].徐州工程学院学报（自然科学版）,2023,38(4):5-13,9.基金项目
国家自然科学基金项目(82060645) （82060645）
上海市科技兴农重点攻关资助项目(沪农科创字(2018)第1-1号) （沪农科创字(2018）
