西北师范大学学报（自然科学版）2024，Vol.60Issue(1)：61-69,9.DOI:10.16783/j.cnki.nwnuz.2024.01.010
LED3A强化紫花苜蓿修复Cd污染土壤的效果研究
Effect of LED3 A enhanced phytoremediation of cadmium contaminated soil by alfalfa
摘要
Abstract
A biodegradable chelating agent named N-lauroyl ethylenediamine triacetate(LED3 A)was applied to the induction-enhanced phytoremediation of Cd contaminated soil by alfalfa.The pot experiments were conducted to investigate the effect of LED3 A on the physiological indexes of alfalfa,the variations in subcellular distribution and chemical forms of Cd in plants,as well as the performance of LED3 A in assisting phytoextraction of Cd from soil.The results showed that Cd stress significantly inhibits the plant growth and photosynthesis,and increases malondialdehyde(MDA)accumulation.After application of LED3 A into soil-cultivated system,the biomass,photosynthetic pigments content,peroxidase(POD)and superoxide dismutase(SOD)activities of alfalfa all increase.Changes in these indexes can alleviate cellular membrane lipid peroxidation damage and oxidative stress caused by Cd.LED3 A's chelating enhancement effect results in a higher proportion of Cd in the cytoplasm of alfalfa cells,facilitates the transformation of Cd from highly active forms to less active forms within the plant,improves Cd resistance and reduces toxicity through cell wall immobilization and vacuole compartmentalization.Compared with the treatment without LED3 A,the total Cd content and accumulation in alfalfa at optimal LED3 A dosage(321.3 mg·kg-1)are increased by 29.4%and 69.1%.The bioconcentration factor(BCF)and translocation factor(TF)of Cd in alfalfa root and shoot increase correspondingly by 28.6%,36.4%and 10.5%,respectively.In conclusion,the application of situable amount of LED3 A can improve the Cd tolerance of alfalfa and enhance the efficiency of phytoremediation for Cd contaminated soil.关键词
LED3A/紫花苜蓿/Cd污染土壤/植物修复/化学强化Key words
LED3 A/alfalfa/Cd contaminated soil/phytoremediation/chemical enhancement分类
资源环境引用本文复制引用
刁静茹,肖奇赞,张淋淋,闫利甜,赵保卫..LED3A强化紫花苜蓿修复Cd污染土壤的效果研究[J].西北师范大学学报（自然科学版）,2024,60(1):61-69,9.基金项目
国家自然科学基金资助项目(41261077) （41261077）
甘肃省高等学校创新基金项目(2021B-102) （2021B-102）
