医学信息2024，Vol.37Issue(1)：140-144,5.DOI:10.3969/j.issn.1006-1959.2024.01.024
转染 GKN2 基因对 CD44(+)SGC7901胃癌干细胞的抑制作用
Inhibitory Effect of GKN2 Gene Transfection on CD44(+)SGC7901 Gastric Cancer Stem Cells
胡皓彬 1凌晖 1蔡志宏 1肖萍 1李艳春1
作者信息
- 1. 湖南省人民医院/湖南师范大学附属第一医院病理科,湖南 长沙 410002
- 折叠
摘要
Abstract
Objective To investigate the effect of high expression of GKN2 on the proliferation,migration and invasion of CD44(+)SGC7901 gastric cancer stem cells(GCSCs).Methods CD44(+)SGC7901 GCSCs were isolated from human gastric cancer cell line SGC7901 by magnet-activated cell sorting(MACS),and the sorting efficiency was tested by immunofluorescence.The GKN2 high expression adenovirus vector was transfected into GCSCs,and the expression of GKN2 after transfection was verified by Western Blot.The effects of high expression of GKN2 on the proliferation,migration and invasion of human GCSCs were observed by CCK8 and Transwell migration and invasion experiments.Results Immunofluorescence results showed that the number of CD44(+)SGC7901 GCSCs increased significantly after magnet-activated cell sorting.CCK8 proliferation assay showed that the OD value of GKN2 transfection group at 450 nm was lower than that of non-transfection group and empty vector group(P＜0.05).Transwell migration assay showed that the number of cells migrating and invading to the lower chamber in the GKN2 high expression group was lower than that in the non-transfection group and the empty vector group(P＜0.05).Conclusion High expression of GKN2 can inhibit the proliferation,migration and invasion of CD44(+)SGC7901 GCSCs.关键词
胃癌/胃癌干细胞/GKN2Key words
Gastric cancer/Gastric cancer stem cells/GKN2分类
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胡皓彬,凌晖,蔡志宏,肖萍,李艳春..转染 GKN2 基因对 CD44(+)SGC7901胃癌干细胞的抑制作用[J].医学信息,2024,37(1):140-144,5.
