应用化学2023，Vol.40Issue(11)：1587-1594,8.DOI:10.19894/j.issn.1000-0518.230147
UPLC-Q Exactive Plus-MS分析高粱壳化学成分及黄嘌呤氧化酶体外抑制活性筛选
Chemical Analysis of Sorghum Husk by UPLC-Q Exactive Plus-MS in Vitro Screening of Xanthine Oxidase Inhibitory Activity and Composition
摘要
Abstract
The chemical components in sorghum husk are analyzed and identified by high performance liquid chromatography tandem with quadrupole electrostatic field orbital trap mass spectrometry and screened for inhibition of xanthine oxidase activity components by high performance liquid chromatography.The experiment is performed on a Hypersil GOLD C18 column(2.1 nm×30 nm,3 μm)with a gradient elution of 0.1％formic acid water(A)-acetonitrile(B)at a flow rate of 1.0 mL/min and a sample volume of 10 μL.The mass spectrometry analysis is performed using an ESI ion source and data are collected in positive ion mode.The data obtained are analyzed using Xcalibur 4.1 software in combination with a database,and a total of 27 chemical components are identified.Six chemical components,namely acacetin,puerarin,apigenin,phlorizin,isorhamnetin and fisetin,are screened for activity,and apigenin and fisetin are found to exhibit strong inhibitory activity on xanthine oxidase(XO)with IC50 of 18.11 and 34.59 μmol/L,respectively.关键词
高粱壳/化学成分鉴定/质谱碎裂机理/黄嘌呤氧化酶/抑制活性成分Key words
Sorghum husk/Chemical composition identificationXanthine/Mass Spectrometry Fragmentation Mechanisms/Xanthine oxidase/Inhibition of active ingredients分类
化学化工引用本文复制引用
张瀚水,龚本义,南敏伦,赫玉芳,赵昱玮,吴丛梅,殷玉和..UPLC-Q Exactive Plus-MS分析高粱壳化学成分及黄嘌呤氧化酶体外抑制活性筛选[J].应用化学,2023,40(11):1587-1594,8.基金项目
吉林省科技发展计划项目(No.20220204014YY)资助 Supported by Jilin Province Science and Technology Development Plan Project(No.20220204014YY) （No.20220204014YY）
