基于公共数据库分析FOXM1在软组织肉瘤中的表达及临床预后意义OA

Objective To analyze the expression and clinical prognostic value of forkhead box M1(FOXM1)in soft tissue sarcomas(STSs).Methods The expression level of FOXM1 in common malignant tumors,especially STSs,was analyzed by Oncomine database,and then verified in TCGA database.The expression level of FOXM1 in STSs cell lines was analyzed in CCLE database,and the difference of FOXM1 expression in STSs with gender,race and age groups was analyzed in UALCAN database.The relationship between FOXM1 and the prognosis of STSs patients and its correlation with the expression of key molecules of PI3K/AKT signaling pathway in STSs were analyzed by GEPIA database.The interaction network of FOXM1 interacting proteins was analyzed in the STRING database.Finally,GO functional annotation and KEGG pathway enrichment analysis were performed for FOXM1 and its interacting genes using the online DAVID tools.Results A total of 9 studies in the Oncomine database involved the expression of FOXM1 in STSs tissues and corresponding adjacent tissues,and FOXM1 was highly expressed in STSs(P＜0.05).The verification results of the TCGA database were consistent with the mining results of the Oncomine database.FOXM1 was generally highly expressed in STSs cells(P＜0.05),and the expression level of FOXMl was related to gender,but not to race and age.GEPIA database analysis showed that the overall survival(OS)and disease-free survival(DFS)of patients with low expression of FOXM1 were higher than those of patients with high expression(P＜0.05).The expression level of FOXM1 in STSs tissues was positively correlated with the expression levels of phosphatidylinositol 3-kinase catalytic subunit α(PIK3CA),AKT1 and mammalian target of rapamycin(mTOR)(r=0.26,0.43,R=0.42).Protein interaction network analysis showed that CCNB1,PLK1,CDK1,CCNA2,MYBL2,CCNB2,CENPF,CDK2,CDC25A,CDC20 and other proteins had obvious interaction with FOXM1.GO enrichment analysis showed that these genes involved in biological functions including cell division and cell cycle regulation.KEGG enrichment analysis showed that these genes were mainly involved in signaling pathways including p53 signaling pathway,FOXO signaling pathway and PI3K-Akt signaling path way.Conclusion FOXM1 is highly expressed in soft tissue sarcoma and is related to the prognosis of patients with soft tissue sarcoma.The mechanism may be related to the regulation of PI3K/AKT signaling pathway.