医学信息2024，Vol.37Issue(1)：10-15,6.DOI:10.3969/j.issn.1006-1959.2024.01.002
基于m6A相关长链非编码RNA鉴定克罗恩病的潜在诊断生物标志物
Identification of Potential Diagnostic Biomarkers for Crohn's Disease Based on m6A-related Long Non-coding RNAs
摘要
Abstract
Objective To explore the long non-coding RNA(LncRNA)associated with m6A in Crohn's disease(CD)and its correlation with clinical characteristics of patients.Methods The CD chip data was obtained through the GEO database to obtain the expression of m6A-related methylase,and the LncRNA was screened by differential analysis.The LncRNA co-expressed with m6A methylase was analyzed to explore the correlation between LncRNA and the disease activity of CD patients and draw the ROC curve.GO enrichment analysis and KEGG signaling pathway analysis were performed on the mRNA co-expressed with LncRNA.Results A total of 7 LncRNAs related to m6A in CD were screened,and 3 LncRNAs were correlated with 8 m6A methylases.Among them,LncRNAGSEC was positively correlated with disease activity index CDEIS.The prediction of m6A methylation site suggested that there were multiple hypermethylation sites in GSEC.The area under the ROC curve was 0.805.GO enrichment analysis showed that it was enriched in protein serine/threonine/tyrosine kinase activity,Toll-like receptor binding and other functions.KEGG signaling pathway analysis showed that it was enriched in NF-κB signaling pathway,inflammatory bowel disease and other pathways.Conclusion GSEC may be a potential diagnostic and therapeutic target for CD.关键词
克罗恩病/m6A/LncRNA/GSECKey words
Crohn's disease/m6A/LncRNA/GSEC分类
临床医学引用本文复制引用
黄晓东,黄衔,梁靖华,李昂,刘琦凯,钟秀清,肖剑伟..基于m6A相关长链非编码RNA鉴定克罗恩病的潜在诊断生物标志物[J].医学信息,2024,37(1):10-15,6.基金项目
1.广东省中医药管理局中医药科研项目(编号:20221342) （编号:20221342）
2.深圳市福田区卫生公益性科研项目(编号:FTWS2020046) （编号:FTWS2020046）
