中国农业科学2023，Vol.56Issue(23)：4635-4647,13.DOI:10.3864/j.issn.0578-1752.2023.23.006
棉花功能基因图位克隆的研究进展
Research Advances of Map-Based Cloning Genes in Cotton
摘要
Abstract
Map-based cloning is a classical and effective method to identify candidate genes for specific phenotypic variants.Map-based cloning of functional genes plays important roles in the innovative utilization of germplasm resources,molecular design breeding and improving breeding efficiency.In recent years,the whole-genome sequencing of Gossypium raimondii,Gossypium arboreum,Gossypium hirsutum,and Gossypium barbadense has been completed and improved.Map-based cloning has entered into a crucial period.In 2016,the dominant glandless gene Gl2e(GoPGF)was the first map-based cloning gene in cotton.So far,20 qualitative traits genes and 5 quantitative traits genes have been identified by map-based cloning technology.In this paper,research progress was systematically reviewed in fiber,gland,nectary,leaf type,plant architecture,plant color,and fertility in terms of gene symbols,names,chromosomal positioning,and candidate genes.Moreover,map-based cloning strategies were systematically reviewed in mapping populations and bulked segregate analysis-sequencing(BSA-seq).With the reduction of sequencing cost and utilization of BSA-seq,it is believed that more and more genes will be cloned by map-based cloning technology.In addition,transformation and genome editing have been successfully used to evaluate the function of the candidate gene in the target interval.It is believed that map-based cloning could provide a theoretical basis and genetic resources for molecular design breeding in cotton.关键词
棉花/图位克隆/分子标记/作图群体/BSA-seqKey words
cotton/map-based cloning/molecular marker/mapping population/BSA-seq引用本文复制引用
臧新山,高宇,王星星,彭云玲,马雄风,王康文,张先亮,王雪平,王军,梁雨,裴小雨,任翔,吕宇龙..棉花功能基因图位克隆的研究进展[J].中国农业科学,2023,56(23):4635-4647,13.基金项目
国家棉花产业技术体系(CARS-15-07)、新疆维吾尔自治区重点研发任务专项(2022B02052-2)、新疆维吾尔自治区天山英才计划(2021)、昌吉回族自治州科技重大专项(2021Z01-01) （CARS-15-07）
