中国农业科学2023，Vol.56Issue(24)：4814-4825,12.DOI:10.3864/j.issn.0578-1752.2023.24.002
小麦穗长QTL鉴定及其遗传分析
Identification and Genetic Analysis of QTL for Spike Length in Wheat
摘要
Abstract
[Objective]Spike length(SL)plays an important role in determining spike structure and yield potential of wheat.Quantitative trait loci(QTL)for spike length were excavated and their genetic effects were further analyzed to provide theoretical basis for molecular breeding.[Method]This study consisted of a population of 198 F6 recombinant inbred lines(RIL)derived from the cross between the natural mutant msf and the cultivar Chuannong 16(MC population).The MC population and its parents were planted in five different environments including Wenjiang in 2021 and 2022(2021WJ and 2022WJ);Chongzhou in 2021 and 2022(2021CZ and 2022CZ);and Ya'an in 2021(2021 YA)for spike length measurement.The 16K SNP chip-based constructed high-quality and high-density genetic linkage maps were used to map QTL for spike length.Additionally,the genotype of the flanking markers for the major spike length QTL was used to analyze its genetic effect on yield-related traits and thus to evaluate its potentiality for yield improvement.[Result]A total of 14 QTL for spike length were identified and they were mainly distributed on chromosomes 1A(one),1B(one),2B(one),3D(three),4A(one),4D(two),5A(one),5B(one),7A(one),7B(one),and 7D(one).Among them,QSl.sau.1A was detected in four environments and the best linear unbiased prediction(BLUP)value,explained 6.46％to 20.12％of the phenotypic variation,and thus was regarded as a major QTL.The positive allele at QSl.sau.1A came from the parental line msf.QTL analysis across multiple environments also detected QSl.sau.lA,indicating it exhibits minimal environmental influence and represents a major and stably expressed QTL.The effect of QSl.sau.lA was successfully verified in two populations with different genetic backgrounds.Genetic effects analysis showed that the positive allele of QSl.sau.lA showed a significant effect on improving grain number per spike(12.68％),grain weight per spike(14.99％),1000-grain weight(5.79％),flag leaf width(2.94％),spikelet number(1.48％),and flowering date(0.61％),and a significant effect of reducing plant height(-6.47％)and effective tiller number(-36.11％).[Conclusion]A major and stably expressed spike length QTL,QSl.sau.1A,was detected on chromosome 1 A.Its positive allele significantly increased grain number per spike,grain weight per spike,thousand grain weight,and spikelet number per spike,indicating its great breeding value.关键词
小麦/16K SNP芯片/QTL/穗长/产量Key words
wheat/16K SNP array/QTL/spike length/yield引用本文复制引用
姚琦馥,崔凤娟,蒋云,马建,周界光,王健,陈黄鑫,杨瑶瑶,刘倩,闫磊,王瑛,周景忠..小麦穗长QTL鉴定及其遗传分析[J].中国农业科学,2023,56(24):4814-4825,12.基金项目
贵州省科技计划基础研究项目(黔科合基础-ZK[2021]一般131)、铜仁市科技计划基础研究项目(铜市科研[2023]36号) （黔科合基础-ZK[2021]一般131）
