中国农业科学2023，Vol.56Issue(24)：4842-4853,12.DOI:10.3864/j.issn.0578-1752.2023.24.004
不同类型地膜对花生生长及根际细菌群落影响的比较分析
Comparative Analysis of the Effects of Different Types of Plastic Film on Peanut Growth and Rhizobacterial Community
摘要
Abstract
[Objective]This study was carried out to investigate the effects of different types of plastic film on peanut rhizobacterial community and peanut growth/yield,explore the relationships between rhizobacterial community and peanut growth/yield,and provide a basis for further improving peanut yield.[Method]Four treatments were set up and conducted for 9 continuous years,including control treatment(LU),black film treatment(HEI),bio-degradable film treatment(JI)and conventional film treatment(PU).Taking the rhizosphere soil of peanut at the flowering stage and mature stage in 2022 as the research object,Illumina MiSeq PE300 as the sequencing platform,and 16S rRNA gene as the target gene,effects of different types of film treatments on the structure and function of rhizobacterial community and the growth and yield of peanut were studied.[Result]Compared with LU,peanut yield was increased by-0.45％-2.34％,2.44％-14.36％and 6.14％-24.69％in HEI,JI and PU treatment,respectively.At the flowering stage and mature stage,HEI treatment totally altered the relative abundances of 6 rhizobacterial communities at the levels of class and genus,while results of correlation analysis showed that there was no correlation between rhizobacterial community and peanut growth or yield.JI treatment totally altered 6 rhizobacterial communities at the levels of class and genus,among which,1 class(Blastocatellia)showed positive relationship with peanut yield.PU treatment totally influenced 12 rhizobacterial communities at the levels of class and genus,of these,1 class(Acidobacteriae)and 1 genus(Ellin6067)were correlated with peanut main stem height and lateral branch number at the flowering stage,1 class(Clostridia)and 2 genera(Pseudomonas,Ellin6067)were correlated with peanut yield at the mature stage.The functional prediction results of rhizobacterial community showed that HEI treatment reduced the nitrogen and nitrate respiration functions at the flowering stage and mature stage;JI treatment reduced the functions of nitrate reduction,nitrogen respiration and nitrate respiration at the flowering stage,but had no significant effect on nitrogen metabolism at the mature stage;PU treatment decreased aerobic ammonia oxidation and nitrate respiration at the flowering stage,and increased these two nitrogen metabolism functions at the mature stage.[Conclusion]Black film has no significant effect on peanut yield and rhizobacterial community;Bio-degradable film can improve the structure of rhizobacterial community and yield at the mature stage,but its yield increasing effect is not stable;Conventional film possesses better improving effects than bio-degradable film in peanut yield and rhizobacterial community structure and function.关键词
黑膜/降解膜/普通膜/花生/根际细菌群落Key words
black film/bio-degradable film/conventional film/peanut/rhizobacterial community引用本文复制引用
于天一,吴月,杨吉顺,吴正锋,张智猛,沈浦,郑永美,李尚霞,吴菊香,孙棋棋..不同类型地膜对花生生长及根际细菌群落影响的比较分析[J].中国农业科学,2023,56(24):4842-4853,12.基金项目
山东省自然科学基金(ZR2022QC005)、山东省农业科学院创新工程(CXGC2023A46,CXGC2023A22)、中央引导地方科技发展项目(YDZX2022131) （ZR2022QC005）
