中国农业科学2023，Vol.56Issue(24)：4854-4865,12.DOI:10.3864/j.issn.0578-1752.2023.24.005
氧酰基载体蛋白还原酶基因FgOAR1对禾谷镰孢生长、发育和致病的影响
The Effect of 3-Oxyacyl ACP Reductase Gene FgOAR1 on the Growth,Development and Pathogenicity of Fusarium graminearum
摘要
Abstract
[Background]Fusarium graminearum is the main pathogenic fungus which can infect wheat and result in Fusarium head blight.The infection of F.graminearum leads to huge reduction in crop quality and yield,and produces different types of mycotoxins that endanger grain production and human health.3-oxoacyl ACP reductase(OAR1),catalyzing carboxyacyl carrier protein to oxyacyl carrier protein,which plays an important role in the fatty acid biosynthesis of F graminearum.[Objective]To reveal the biological function of FgOAR1 in F.graminearum,the FgOAR1 deletion mutants were constructed.The phenotype,cell structure,conidia concentration,sexual reproduction and pathogenicity of mutant were analyzed and compared with wild-type strain PH-1.The results will uncover the function of FgOAR1 in the growth and pathogenic process of F graminearum,and provide scientific evidences for the identification of novel antifungal target and control of Fusarium head blight.[Method]Wild-type strain F.graminearum PH-1 was used as material in this study.Split-Marker gene knockout technology was conducted to construct the△FgOAR1 mutants.The △FgOAR1-C reverting strain was obtained by the Gap-repair method.All strains were individually inoculated in common media(PDA,CM,YPG),and the stress selection medium containing Congo-Red,SDS,NaCl and H2O2,respectively.The mycelium phenotypes and fatty acid content were recorded.The strains were inoculated in CMC medium to analyze the conidia concentration.The carrot culture-medium was used to analyze the sexual reproduction of each strain.All strains were individually inoculated to the wheat coleoptile and spikelet to analyze the pathogenicity and mycotoxin content,respectively.The disease incidence of mutant strains was calculated and compared with PH-1 strain.[Result]Compared with the wild-type strain PH-1,the growth of △FgOAR1 mutant showed no difference in PDA,YEG and CM media.Whereas,under the pressure of 0.7 mol·L-1 NaCl,0.03％H2O2,0.01％SDS and 300 μg·mL-1 Congo-Red,the growth of △FgOAR1 mutant was significantly reduced compared with PH-1.The evidence demonstrated that FgOAR1 is related to the cell membrane and cell wall formation in F graminearum.In CMC medium,the conidia concentration of △FgOAR1 mutant(8.1 × 105 conidia/mL)was less than PH-1(1.26× 106 conidia/mL)with significant difference;The pathogenicity analysis further demonstrated that the disease incidence of△FgOAR1 mutant was significantly lower than PH-1 in wheat coleoptile and spikelet inoculation tests.[Conclusion]FgOAR1 participates in the fatty acid biosynthesis,and plays an important role in cell membrane formation of F graminearum.The deletion of FgOAR1 resulted in the reduced resistance to pressures,decreased conidia production and pathogenicity.Therefore,FgOAR1 is important for the growth,development and pathogenicity process of F.graminearum.关键词
禾谷镰孢/小麦赤霉病/基因敲除/FgOAR1/致病力Key words
Fusarium graminearum/Fusarium head blight/gene knockout/FgOAR1/pathogenicity引用本文复制引用
宫安东,雷银玉,吴楠楠,刘景榕,宋梦鸽,张艺美,杨光,杨鹏..氧酰基载体蛋白还原酶基因FgOAR1对禾谷镰孢生长、发育和致病的影响[J].中国农业科学,2023,56(24):4854-4865,12.基金项目
国家自然科学基金(31701740)、河南省自然科学基金(222300420519,222301420111)、河南省教育厅高等学校重点科研项目(24A210025)、河南省科技攻关项目(232102110202)、南湖学者奖励计划 （31701740）
