中国药房2024，Vol.35Issue(1)：38-43,6.DOI:10.6039/j.issn.1001-0408.2024.01.07
基于代谢组学研究蒙药苏格木勒-4对失眠大鼠的改善作用机制
Metabolomics-based study on the improvement mechanism of the Mongolian drug Sugemule-4 on insomnia rats
摘要
Abstract
OBJECTIVE To study the effects of the Mongolian medicine Sugemule-4 on the metabolism of insomnia rats,and to preliminarily explore its possible mechanisms for improving insomnia.METHODS The rat model of chronic stress insomnia was established by tail clipping stimulation and intraperitoneal injection of p-chlorophenyl alanine solution.Twenty-four male rats were randomly divided into the normal group,model group,diazepam group(positive control,0.92 mg/kg),and Sugemule-4 group(5.2 g/kg),with 6 rats in each group.Since the 7th day of tail clipping stimulation,the Sugemule-4 group and diazepam group began to be intragastrically administered with relevant medicine;the normal group and model group were intragastrically administered with an equal volume of distilled water,once a day,for 14 consecutive days.The learning and memory abilities of rats were tested using a water maze experiment,and the non-invasive sleep activity monitoring system was used to monitor the 24-hour sleep time of rats.A metabolomics study was conducted on rat serum and hippocampal tissue by using ultra-high-performance liquid chromatography-tandem mass spectrometry.The multivariate statistical analysis method was adopted to analyze the differential metabolites in serum and hippocampal tissue of rats,and screen for differential metabolites and metabolic pathways among those groups.RESULTS Compared with the normal group,the escape latency of rats in the model group was significantly increased,the times of crossing platforms were significantly reduced,and the percentage of average 24-hour sleep time was significantly reduced(P＜0.05).Compared with the model group,the levels of the above indicators were significantly reversed in the diazepam group and Sugemule-4 group(P＜0.05).Metabolomics studies found that a total of 9 differential metabolites were identified in rat serum and hippocampal tissue,including 5-hydroxyindoleacetic acid,canine urate,canine urinary quinolinic acid,5-hydroxytryptamine,phenol sulfate,1-carboxyethyltyrosine,3-(4-hydroxyphenyl)lactate,N-acetyl tyrosine,tyrosine and phenol sulfate,mainly involving 2 metabolic pathways of tryptophan and tyrosine.CONCLUSIONS Sugemule-4 can improve the sleep time and behavioral performance of insomnia rats,and its mechanism may be associated with affecting amino acid metabolic pathways such as tryptophan and tyrosine.关键词
蒙药/苏格木勒-4/失眠/代谢组学/氨基酸代谢Key words
Mongolian medicine/Sugemule-4/insomnia/metabolomics/amino acid metabolism分类
医药卫生引用本文复制引用
李艳佳,杨蕊,王昇,孙利东,白东浩,靳尚武..基于代谢组学研究蒙药苏格木勒-4对失眠大鼠的改善作用机制[J].中国药房,2024,35(1):38-43,6.基金项目
内蒙古自治区科技计划项目(No.2019GG120) （No.2019GG120）
