中国药房2024，Vol.35Issue(1)：57-62,6.DOI:10.6039/j.issn.1001-0408.2024.01.10
茯苓酸改善多囊卵巢综合征大鼠胰岛素抵抗的作用及机制研究
Improvement effects of poria acid on insulin resistance in rats with polycystic ovary syndrome and its mechanism
摘要
Abstract
OBJECTIVE To study the improvement effects of poria acid on insulin resistance in rats with polycystic ovary syndrome(PCOS)and its mechanism.METHODS One hundred and twenty-six female rats were randomly separated into blank group,PCOS group,poria acid low-dose group(8.33 mg/kg),pachymic acid high-dose group(33.32 mg/kg),ethinylestradiol cyproterone group(positive control group,0.34 mg/kg),recombinant rat high mobility group protein B1 protein(rHMGB1)group(8 μg/kg),and poria acid high dose+rHMGB1 group(33.32 mg/kg poria acid+8 μg/kg rHMGB1),with 18 rats in each group.Except for the blank group,the rats in all other groups were given Letrozole suspension intragastrically to construct the PCOS model.After successful modeling,administration was performed once a day for 4 weeks.After medication,the fasting blood glucose and fasting insulin levels,and insulin resistance index(HOMA-IR)were measured in rats;the levels of follicle-stimulating hormone(FSH),luteinizing hormone(LH)and testosterone(T)in rat serum,and the levels of interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)in ovarian tissue were detected;ovarian coefficients of rats were calculated;the pathological changes of ovarian tissue were observed;the expressions of HMGB1,receptor for advanced glycosylation end product(RAGE)and phosphorylated nuclear factor κB p65(p-NF-κB p65)proteins were determined in ovarian tissue of rats.RESULTS Compared with the blank group,the pathological injury of ovarian tissue of rats in the PCOS group was serious,the levels of fasting blood glucose and fasting insulin,HOMA-IR and ovarian coefficient were increased,the levels of serum LH and T were increased,while the levels of FSH were decreased;the levels of IL-1β and TNF-α,the expressions of HMGB1,RAGE and p-NF-κB p65 protein in ovarian tissue were increased,with statistical significance(P＜0.05).Compared with the PCOS group,pathological damage of ovarian tissue was reduced in poria acid low-dose and high-dose groups and ethinylestradiol cyproterone group,and fasting blood glucose,fasting insulin levels,HOMA-IR and ovarian coefficient were decreased;serum LH and T levels were decreased,while FSH levels were increased;the levels of IL-1β and TNF-α and the expressions of HMGB1,RAGE and p-NF-κB p65 protein in ovarian tissue were decreased,with statistical significance(P＜0.05).The trend of corresponding indexes in rHMGB1 group was opposite to the above(P＜0.05).Compared with poria acid high-dose group,the changes of the above indexes were reversed significantly in poria acid high-dose+rHMGB1 group(P＜0.05).CONCLUSIONS Poria acid may improve insulin resistance and inhibit inflammatory reaction in PCOS rats by inhibiting HMGB1/RAGE pathway.关键词
茯苓酸/多囊卵巢综合征/高迁移率族蛋白B1/晚期糖基化终产物受体通路/胰岛素抵抗/炎症反应Key words
poria acid/polycystic ovary syndrome/HMGB1/RAGE pathway/insulin resistance/inflammation response分类
医药卫生引用本文复制引用
唐虹,李林霞,华宇,蒋晓梅,章仕雯,付金荣..茯苓酸改善多囊卵巢综合征大鼠胰岛素抵抗的作用及机制研究[J].中国药房,2024,35(1):57-62,6.基金项目
全国中医学术流派传承工作室第二轮建设项目(No.国中医药人教函[2019]62号) （No.国中医药人教函[2019]62号）
上海市浦东新区科技发展基金事业单位民生科研专项(医疗卫生项目)(No.PKJ2022-Y09) （医疗卫生项目）
上海市第七人民医院"名中医继承人"人才培养计划项目(No.JCR2021-02) （No.JCR2021-02）
