中国实验动物学报2023，Vol.31Issue(11)：1399-1407,9.DOI:10.3969/j.issn.1005-4847.2023.11.003
新型大鼠腰部慢性骨骼肌复合损伤模型的建立
Establishment of a novel composite rat model of chronic skeletal muscle injury
摘要
Abstract
Objective To establish a novel rat model of chronic skeletal muscle composite injury in the lumbar region,to explore its mechanism and treatment method in depth.Methods Twenty-eight SD rats were randomly divided into control group(n = 10),Hitting injury model(HIM)group(n = 9),Composite damage model(CDM)group(n = 9)(vertical blow combined with forced prolonged sitting).Open field test,HE staining and PCR sequencing were performed 3 days after modeling.Results The open field distance of rats in HIM group was significantly shorter than that in the CDM group,and both groups had shorter distances than the control group.HE staining showed multiple muscle fiber breaks,significant widening of muscle gaps,uneven distribution of muscle nuclei,and visible congestion in the model group of simple blow injury;In the CDM group,muscle fibers were ruptured,the muscle gaps were slightly widened,and inflammatory cell infiltration was observed between the muscles,especially around the muscle fiber breakage,with muscle fiber nuclei gathering.A volcano plot of the sequencing result suggested significant differences in the Pf4 gene.GO analysis of muscle tissue in model group rats showed upregulation of oxygen binding and carrier activity pathways.Conclusions This study attempted to establish a composite skeletal muscle injury model to study the pathological changes of chronic skeletal muscle injury in the low back pain and evaluate the efficacy of treatment method.It has practical application value in animal experiments for chronic non-specific low back pain.关键词
大鼠模型/腰部慢性骨骼肌损伤/转录组测序/复合损伤Key words
rat model/chronic skeletal muscle injury/transcriptome sequencing/composite injury分类
生物科学引用本文复制引用
卢晶晶,张弛,张驰名,许铛瀚,林煜翔,谢炜星..新型大鼠腰部慢性骨骼肌复合损伤模型的建立[J].中国实验动物学报,2023,31(11):1399-1407,9.基金项目
国家中医药传承创新中心科研专项(2022QN26),广东省中医针灸重点实验室开放基金科研项目(浮针专项:01),广东省中医药管理局科研平台专项(20244007).Funded by the National Traditional Chinese Medicine Inheritance and Innovation Center Research Special Project(2022QN26),Key Laboratory of Guangdong Provincial for Acupuncture and Chinese Medicine(Special Project for Floating Acupuncture:01),Guangdong Provincial Bureau of Traditional Chinese Medicine Research Project(20244007). （2022QN26）
