中国比较医学杂志2023，Vol.33Issue(12)：1-6,6.DOI:10.3969/j.issn.1671-7856.2023.12.001
不同月龄C57BL/6小鼠大脑皮质功能区的微血管形态计量学分析
Microvascular morphometric analysis of cerebral cortical functional areas in C57BL/6 mice of various ages
摘要
Abstract
Objective To observe and analyze functional areas of the cerebral cortex in C57BL/6 mice of various ages.Methods Improved alkaline phosphatase staining was used to reveal the microvascular morphology of the cerebral cortex in C57BL/6 mice,including the motor cortex(primary and secondary motor cortex),sensory cortex(primary and secondary somatosensory cortex),visual cortex(primary and secondary visual cortex),and auditory cortex(primary and secondary auditory cortex),olfactory cortex(extrarhinal and entorhinal cortex).Images were captured under an OLYMPUS BX51 microscope with Image-Pro Plus 5.1 software.The microvascular length density(Lv),microvascular surface area density(Sv),and microvascular volume density(Vv)were analyzed by Image-Pro Plus 5.1 software.Results Expression of alkaline phosphatase was abundant in cerebral cortical microvessels of adult and elderly mice,and slightly expressed in juvenile mice,but not in lactating mice.Pial blood vessels enter the cortex in T shape,Y shape,large arc,and small arc four manners.Lv,Sv and Vv in different parts of the same aged mice showed a decreasing trend in motor,sensory,visual,auditory and olfactory cortexes,and the microvascular density of Lv,Sv and Vv in motor and sensory cortexes was statistically significant compared with the olfactory cortex(P<0.05).The vascular density in all functional areas in elderly mice was lower than that in adult mice,but no statistical significance was found(P>0.05).Conclusions The expression of alkaline phosphatase in microvessels in functional areas of the cerebral cortex in C57BL/6 mice increases with age and reached its peak value in adulthood.The microvascular architecture in the brain provides morphological parameters to establish cerebrovascular disease models.关键词
碱性磷酸酶染色/C57BL/6小鼠/大脑皮质/微血管构筑Key words
alkaline phosphatase staining/C57BL/6 mice/cerebral cortex/microvascular construction分类
医药卫生引用本文复制引用
郭民,郭永昌,张瑞虎,张引红,王海龙,陈朝阳..不同月龄C57BL/6小鼠大脑皮质功能区的微血管形态计量学分析[J].中国比较医学杂志,2023,33(12):1-6,6.基金项目
国家重点研发计划项目(2021YFF0702805). （2021YFF0702805）
