中国比较医学杂志2023，Vol.33Issue(12)：21-27,7.DOI:10.3969/j.issn.1671-7856.2023.12.004
人羊膜上皮细胞移植对子宫瘢痕模型大鼠子宫内膜的影响及机制
Effect and mechanism of transplantation of human amniotic epithelial cells on endometrium of uterine scar model rats
摘要
Abstract
Objective To investigate the effect of human amniotic epithelial cell(hAEC)transplantation on endometrium improvement and matrix metalloproteinase 8(MMP-8)and vascular endothelial growth factor(VEGF)expression in a rat model of uterine scaring.Methods The uterine scar model was established in rats that were randomly divided into model and transplantation groups with 18 rats in each group.The other 18 rats were used as the sham operation group.Rats in the transplantation group were injected with hAECs in the uterine scar,and rats in model and sham operation groups were administered the same amount of PBS.After 4 weeks,the uterine tissues of eight rats in each group were collected.Histomorphological changes and endometria fibrosis were observed by HE staining and Masson staining respectively,and the endometrial thickness and number of glands were measured.Endometrial growth and receptivity were evaluated by immunohistochemical staining of cytokeratin and integrin β3,respectively.mRNA expression of MMP-8 and VEGFA in endometrial tissues was measured by RT-qPCR.Western blot was used to measure MMP-8 and VEGFA protein expression.After 8 weeks,the remaining 10 rats in each group were used to assess gestational ability.Results The endometrial thickness,gland number,IOD value of keratin and integrin β3,relative mRNA and protein expression levels of MMP-8 and VEGFA,pregnancy rate and number of uterine embryos in model and transplantation groups were lower than those in sham operation group(P<0.05).The endometrial thickness,gland number,IOD value of keratin and integrin β3,relative mRNA and protein expression of MMP-8 and VEGFA,pregnancy rate and number of uterine embryos were higher than those in model group(P<0.05).Additionally,hAEC transplantation improved the pathological morphology of endometrial tissue in rats with uterine scaring and reduced the degree of endometrial fibrosis.Conclusions hAEC transplantation improves endometrial injury,reduces scar formation,improves endometrial receptivity,and enhances pregnancy function in model rats,which may be related to promotion of MMP-8 and VEGFA expression.关键词
人羊膜上皮细胞/子宫瘢痕/子宫内膜/基质金属蛋白酶8/血管内皮生长因子AKey words
human amniotic epithelial cells/uterine scar/endometrium/matrix metalloproteinase 8/vascular endothelial growth factor A分类
医药卫生引用本文复制引用
王静,路成吉,臧建新,衣志爽,彭家晟,张峰..人羊膜上皮细胞移植对子宫瘢痕模型大鼠子宫内膜的影响及机制[J].中国比较医学杂志,2023,33(12):21-27,7.基金项目
2020 年河南省医学科技攻关计划项目(LHGJ20200448). （LHGJ20200448）
