中国比较医学杂志2023，Vol.33Issue(12)：28-33,6.DOI:10.3969/j.issn.1671-7856.2023.12.005
恒古骨伤愈合剂对db/db小鼠的降血糖和改善肠道菌群作用研究
Effect of Osteoking on lowering blood sugar and improving intestinal flora in db/db mice
摘要
Abstract
Objective To investigate the effects of Osteoking on hyperglycemia and regulating gut microbiota in db/db mice.Methods Wildtype mice were used as the control group,and db/db mice were randomly divided into model and Osteoking groups.After intragastric administration for 12 weeks,fasting blood glucose and serum glycosylated hemoglobin and insulin levels were measured,changes in intestinal microflora were determined,and functional pathways related to intestinal microflora in mice were predicted by 16S rDNA sequencing.Results Compared with the model group,Osteoking decreased fasting blood glucose(P<0.01),serum glycosylated hemoglobin(P<0.01),and the insulin resistance index(P<0.01),and increased insulin content(P<0.01)in db/db mice.Osteoking increased the abundance of beneficial intestinal microflora and decreased the abundance of harmful bacteria.Moreover,the abundance of Marvinbryantia was increased.Osteoking alleviated the decrease in metabolism of D-arginine and D-ornithine,sphingolipid,and galactose metabolism(P<0.05)and inhibited lysine degradation,the sulfur relay system,and propanoate metabolism(P<0.05).Conclusions Osteoking has hypoglycemic properties and improves the intestinal microflora imbalance in db/db mice.关键词
恒古骨伤愈合剂/降血糖性能/肠道菌群/16S rDNA技术/db/db小鼠Key words
Osteoking/hypoglycemic properties/gut microbiota/16S rDNA sequencing technology/db/db mice分类
医药卫生引用本文复制引用
邓绍友,赵玉兰,王佩锦,李蓉,赵宏斌,角建林,郑红..恒古骨伤愈合剂对db/db小鼠的降血糖和改善肠道菌群作用研究[J].中国比较医学杂志,2023,33(12):28-33,6.基金项目
云南省基础研究-昆医联合专项重点项目(202201AY070001-032) （202201AY070001-032）
云南省创新引导与科技培育计划项目(202204AW050014). （202204AW050014）
