中国比较医学杂志2023，Vol.33Issue(12)：42-48,7.DOI:10.3969/j.issn.1671-7856.2023.12.007
蒙药哈它各其-7通过HIF-1α促进糖尿病大鼠溃疡创面愈合的实验研究
Mongolian drug hatagaqi-7 promotes ulcer wound healing in diabetic rats via HIF-1α
摘要
Abstract
Objective To study the therapeutic value of Mongolian drug hatagaqi-7 for wound healing of diabetic ulcers in rats and preliminarily explore its molecular mechanism in regulating hypoxia-inducible factor-1α(HIF-1α).Methods Adult male SD rats were randomly divided into control,diabetes,Mongolian drug,and cytokine groups.Except in the control group,the other three groups were treated with an intraperitoneal injection of streptozotocin to establish the diabetes model.Ulcer wounds were prepared in the mouse back of the four groups.One week later,the Mongolian drug group was treated with hatagaqi-7,and the cytokine group was treated with recombinant bovine basic fibroblast growth factor for 2 consecutive weeks.Fasting blood glucose(FBG),wound area,wound pathology,expression levels of advanced glycation end products(AGEs),receptor of AGE(RAGE),HIF-1α and vascular endothelial growth factor(VEGF),secreted levels of interleukin-1β(IL-1β),interferon-γ(IFN-γ),and malondialdehyde(MDA),and the total antioxidant capacity(T-AOC)were assessed.Results FBG of diabetes,Mongolian drug and cytokine groups was higher than that in the control group(P<0.05),and no significant difference was observed among the three groups(P>0.05).Compared with the control group,the ulcer wound area,scope of unrepaired tissue,expression levels of AGEs and RAGE,and secreted levels of IL-1β,IFN-γ,and MDA in wound tissue of the diabetes group were increased,and T-AOC and expression levels of HIF-1α and VEGF of the diabetes group were decreased(P<0.05).Compared with the diabetes group,the ulcer wound area,scope of unrepaired tissue,expression levels of AGEs and RAGE,and secreted levels of IL-1β,IFN-γ,and MDA in wound tissue of Mongolian drug and cytokine groups were decreased,T-AOC and expression levels of HIF-1α and VEGF in Mongolian drug and cytokine groups were increased(P<0.05),and indexes of the Mongolian drug group were better than those of the cytokine group.Conclusions Mongolian drug hatagaqi-7 promotes ulcer wound healing in diabetic rats,the inhibiton of AGE and RAGE expression and induction of HIF-1 α are the possible molecular mechanism.关键词
糖尿病溃疡/哈它各其-7/晚期糖基化终末产物/缺氧诱导因子-1αKey words
diabetic ulcer/hatagaqi-7/advanced glycation end products/hypoxia-inducible factor-1α分类
医药卫生引用本文复制引用
董成海,澈力格尔,潮日雅,满柱,包永林,龚利民..蒙药哈它各其-7通过HIF-1α促进糖尿病大鼠溃疡创面愈合的实验研究[J].中国比较医学杂志,2023,33(12):42-48,7.基金项目
内蒙古自治区自然科学基金(2021LHMS08033). （2021LHMS08033）
