中国比较医学杂志2023，Vol.33Issue(12)：49-54,6.DOI:10.3969/j.issn.1671-7856.2023.12.008
MNU诱导SD大鼠下焦湿热证尿血(膀胱癌)模型研究
A model study of blood in urine(bladder cancer)during scorching humid fever in MNU-induced SD rats
摘要
Abstract
Objective To investigate the modeling process and model establishment rate of the N-methyl-N-nitrosourea(MNU)-induced lower jiao dampness-heat syndrome uremia bladder cancer(BC)model.Methods Batch samples were collected by bladder perfusion(MNU).HE staining and pathological observation of pathological tissues were carried out at six time points before,during,and after modeling to understand the formation and development of bladder carcinoma in situ.Results The experimental process was smooth,and no obvious urinary tract bleeding in rats occurred during the operation.Using this modeling technique,considerable bladder tumors were formed after week 8,and obvious epithelial hyperplasia,damage and large-scale tumor formation were observed in the bladder of rats in the model group.Conclusions MNU induces an SD rat bladder cancer model with obvious model establishment and low mortality.The experimental data provide a reference to establish,improve and apply the bladder cancer model.关键词
N-甲基-N-亚硝脲/SD大鼠/膀胱癌/尿血Key words
N-methyl-N-nitrosourea/SD rats/bladder cancer/blood in the urine分类
医药卫生引用本文复制引用
常拓,王洁,李楠,王天奇..MNU诱导SD大鼠下焦湿热证尿血(膀胱癌)模型研究[J].中国比较医学杂志,2023,33(12):49-54,6.基金项目
国家重点研发计划项目(2022YFF0711001) （2022YFF0711001）
辽宁省科协科技创新智库项目(LNKX2022FH02) （LNKX2022FH02）
辽宁中医药大学博士后启动基金(21601A2090). （21601A2090）
