中国医药科学2023，Vol.13Issue(23)：73-76,4.DOI:10.20116/j.issn2095-0616.2023.23.17
沙苑子总黄酮显色条件及提取工艺优选研究
Optimization study on chromogenic conditions and extraction process of total flavonoids from Astragalus complanatus
摘要
Abstract
Objective To optimize the chromogenic and extraction process of total flavonoids from Astragalus complanatus.Methods By using Astragalus complanatus decoction pieces as raw materials,the chromogenic method,optimal chromogenic conditions,extraction method and optimal extraction process of total flavonoids from Astragalus complanatus were studied by single factor combined with orthogonal test.Results AlCl3 method was suitable.The optimal chromogenic conditions were as follows:add 1.2 ml of 60%ethanol,0.6 ml of 2%AlCl3 and 2.0 ml of pH 4.5 acetic acid-sodium acetate buffer to 0.8 ml of the extract,dilute with 60%ethanol to volume,let it stand at room temperature for 20 min,and measure the absorbance at 420 nm.The optimal extraction process was set as follows:70%ethanol,reflux extraction at 85℃ for 2 h,solid-liquid ratio of 1∶25(g/ml),2 extractions in total,and the content of total flavonoids obtained was 24.5 mg/g.Conclusion The chromogenic and extraction method is feasible and reproducible.Therefore,this study provides experimental basis for the further development and utilization of Astragalus complanatus.关键词
沙苑子/总黄酮/显色条件/提取工艺Key words
Astragalus complanatus/Total flavonoids/Chromogenic conditions/Extraction process分类
医药卫生引用本文复制引用
李婷,汪小玉,税丕先,张家伟,石厚银,沈骅睿..沙苑子总黄酮显色条件及提取工艺优选研究[J].中国医药科学,2023,13(23):73-76,4.基金项目
泸州市人民政府-西南医科大学科技战略合作项目(2021LZXNYD-J31) （2021LZXNYD-J31）
四川省中医药管理局中医药科研专项课题(2021MS463). （2021MS463）
