中国药业2023，Vol.32Issue(23)：76-80,5.DOI:10.3969/j.issn.1006-4931.2023.23.016
基于活性成分和α-葡萄糖苷酶抑制作用的组织培养牡荆遗传稳定性评价
Evaluation of Genetic Stability of Tissue-Cultured Viticis Negundo Folium Based on Active Ingredients and Its Inhibitory Effect Against α-Glucosidase
摘要
Abstract
Objective To evaluate the genetic stability of tissue-cultured Viticis Negundo Folium.Methods The extraction effects of four solvents on the total flavonoids from Viticis Negundo Folium were investigated.High-performance liquid chromatography(HPLC)method was used for the content determination of vitexin,and the inhibitory effect of the extract of Viticis Negundo Folium on α-glucosidase and the kinetic curve of vitexin were tested.Results The extraction rates of active ingredients from Viticis Negundo Folium by different solvents were as follows:acidic ethanol(pH 1.33)＞70％ethanol＞acetone＞ethyl acetate.In the tissue-cultured and wild Viticis Negundo Folium,the total flavonoid contents extracted with acidic ethanol as solvent were 62.79 mg/g and 60.35 mg/g(calculated by fresh weight),respectively;the vitexin contents were 132.20 μg/g and 126.12 μg/g(calculated by fresh weight),respectively;the 50％inhibitory concentrations(IC50)of the extract on α-glucosidase were 1.41 mg/mL and 1.37 mg/mL,respectively.The kinetic curve analysis showed that the inhibitory type of the extracts from tissue-cultured and wild Viticis Negundo Folium on α-glucosidase was reversible inhibition.Conclusion The extract of Viticis Negundo Folium has a strong inhibitory effect on α-glucosidase,and the active ingredients of tissue-cultured and wild Viticis Negundo Folium are similar,indicating that tissue-cultured Viticis Negundo Folium has good genetic stability.关键词
牡荆/牡荆素/总黄酮/α-葡萄糖苷酶/抑制作用Key words
Viticis Negundo Folium/vitexin/total flavonoids/α-glucosidase/inhibitory effect分类
医药卫生引用本文复制引用
周梅,孟雪,薛建平,段永波,赵丰兰..基于活性成分和α-葡萄糖苷酶抑制作用的组织培养牡荆遗传稳定性评价[J].中国药业,2023,32(23):76-80,5.基金项目
安徽省高校自然科学研究重点项目[KJ2021A0533]. （）
