中国造纸学报2023，Vol.38Issue(4)：10-18,9.DOI:10.11981/j.issn.1000-6842.2023.04.10
13C-2H双同位素示踪法研究硫酸盐浆中残余木质素的仿酶降解
Study on Biomimetic Degradation of Residual Lignin in Kraft Pulp by 13C-2H Dual Isotope Tracer
摘要
Abstract
In this study,coniferin-[α-13C]was synthesized,and then injected into a growing shoot of Ginkgo biloba L.together with the D-glucose-[6-2H2]of polysaccharide precursor and inhibitor(carboxymethoxyamine hemihydrochloride,AO A)of phenylalanine ammo-nialyas,to obtain 13C and 2H double isotope-labeled ginkgo wood mill.The wood mill was cooked with kraft method and the obtained pulp was treated by enzymatic hydrolysis to obtain 13C and 2H double isotope labeled cellulolytic enzyme lignin(CEL).The purified CEL was subjected to biomimetic treatment with GIF-sur-Yvette(GIF)and Cu(Salen)system which was composed of salicylaldehyde,ethylenediamine and Cu2+to further remove lignin and lignin-carbohydrate complexes(LCC).The residues after biomimetic treatment were analyzed by nuclear magnet-ic resonance(13C NMR and1H NMR).The results showed that the lignin structural units in the residues after GIF system biomimetic treat-ment were mainly connected withβ-5,β-O-4 and β-1 substructures,while a small amount of lignin was connected with cellulose by acetal bond and benzyl ether linkage(about 6.7％).The residual lignin structural units after biomimetic treatment by Cu(Salen)system were mainly connected with β-O-4 and β-1 structures.It was found that there was a small amount of cellulose after the biomimetic treatment in CEL,but no signal of the benzyl ether bond and acetal bond between lignin and cellulose was found.The 1H NMR differential spectrum only showed the signals of some unsubstituted polysaccharides,but the connection between lignin and polysaccharides was not found.The results showed that Cu(Salen)system biomimetic catalytic oxidation had relatively strong degradation ability for LCC.关键词
硫酸盐法蒸煮/残余木质素/双同位素示踪/仿酶降解/银杏Key words
kraft pulping/residual lignin/dual isotope tracer/biomimetic degradation/ginkgo分类
轻工纺织引用本文复制引用
张功侠,路德胜,陈旭东,岳园园,谢益民..13C-2H双同位素示踪法研究硫酸盐浆中残余木质素的仿酶降解[J].中国造纸学报,2023,38(4):10-18,9.基金项目
国家自然科学基金项目(21878070) （21878070）
湖北省高等学校优秀中青年科技创新团队计划项目(T201205). （T201205）
