中国药房2024，Vol.35Issue(1)：15-20,6.DOI:10.6039/j.issn.1001-0408.2024.01.03
梓醇干预PKM2/LDHA表达调控Th17细胞分化的机制研究
Mechanism of catalpol regulating Th17 cell differentiation by interfering PKM2/LDHA expression
摘要
Abstract
OBJECTIVE To investigate the mechanism of catalpol affecting the differentiation of helper T cell 17(Th17)by interfering the expressions of pyruvate kinase M2(PKM2)and lactate dehydrogenase A(LDHA).METHODS The naive CD4+ T cells were selected from the spleen of C57BL/6 mice,and were differentiated into Th17 cells by adding directional differentiation stimulants for 72 hours.At the same time,the cells were treated with 0(directed control),20,40 and 80 μg/mL catalpol.The flow cytometry was used to detect the proportion of Th17 cell differentiation in cells;the colorimetric method was adopted to detect the levels of pyruvate and lactate in cell culture supernatant;mRNA expressions of retinoid-related orphan nuclear receptor gamma t(RORγt),PKM2 and LDHA were detected by qRT-PCR method;Western blot was used to detect the expression levels of PKM2,LDHA,signal transducer and activator of transcription 3(STAT3),and phosphorylated STAT3(p-STAT3)proteins in cells.RESULTS Compared with the directed control group,after 72 hours of treatment with 20,40,80 μg/mL catalpol,the differentiation ratio of Th17 cells were decreased by 6.74%,8.41%,9.24%,and the levels of pyruvate and lactate in the cell culture supernatant,the mRNA expressions of PKM2,LDHA and RORγt as well as the protein expressions of PKM2 and LDHA and the phosphorylation of STAT3 were significantly reduced(P＜0.05).CONCLUSIONS Catalpol can reduce the glycolysis level by down-regulating the expressions of PKM2 and LDHA,thereby inhibiting the differentiation of Th17 cells.关键词
梓醇/糖酵解/辅助性T细胞17/丙酮酸激酶M2/乳酸脱氢酶AKey words
catalpol/glycolysis/helper T cell 17/pyruvate kinase M2/lactate dehydrogenase A分类
医药卫生引用本文复制引用
葛玉,陈雪,王福荣,包宇杰,丁鹏,周玲玲..梓醇干预PKM2/LDHA表达调控Th17细胞分化的机制研究[J].中国药房,2024,35(1):15-20,6.基金项目
国家自然科学基金面上项目(No.82174306) （No.82174306）
