中国药房2024，Vol.35Issue(1)：21-26,6.DOI:10.6039/j.issn.1001-0408.2024.01.04
水冬瓜根及茎的质量控制方法研究
Study on quality control method of the roots and rhizoma of Toricellia angulata
摘要
Abstract
OBJECTIVE To establish the quality control method for the roots and rhizoma of Toricellia angulata.METHODS The properties of the roots and rhizoma of T.angulata were observed and microscopic identification was conducted.The moisture,total ash,acid-insoluble ash and ethanol-soluble extract were examined according to the method stated in the 2020 edition of Chinese Pharmacopoeia(part Ⅳ).HPLC fingerprints of 11 batches of the roots and rhizoma of T.angulata were established,common peaks were identified and the similarity was evaluated by using the Similarity Evaluation System of Chromatographic Fingerprint of TCM(2012 edition).The contents of coniferin,syringin,chlorogenic acid,(+)-syringaresinol-O-β-D-glucopyranoside and syringaresinol were determined by HPLC.RESULTS The properties and microscopic identification of the roots and rhizoma of T.angulata were obvious.The average contents of moisture,total ash,acid-insoluble ash and ethanol-soluble extract were 7.54%,2.18%,0.15%and 7.81%,respectively.There were 16 common peaks marked in the HPLC fingerprints of 11 batches of the roots and rhizoma of T.angulata,with similarities of 0.856-0.960;five of them were identified,such as coniferin,syringin,chlorogenic acid,(+)-syringaresinol-O-β-D-glucopyranoside and syringaresinol.The contents of the above five components were 0.047 2-0.401 6,0.836 8-8.697 9,1.245 3-10.950 0,0.139 0-0.437 8 and 0.016 4-0.635 3 mg/g,respectively.CONCLUSIONS The established method is stable and accurate,which can be used for the quality control of the roots and rhizoma of T.angulata.It is preliminarily proposed that the moisture in the roots and rhizoma of T.angulata is not more than 11.0%,the total ash is not more than 4.0%,the ethanol-soluble extract is not less than 5.0%,the contents of coniferin,syringin,chlorogenic acid,(+)-syringaresinol-O-β-D-glucopyranoside and syringaresinol are not less than 0.04,0.83,1.24,0.13,0.01 mg/g,respectively.关键词
水冬瓜根/水冬瓜茎/性状鉴别/显微鉴别/指纹图谱/含量测定/质量控制Key words
roots of Toricellia angulata/rhizoma of Toricellia angulata/property identification/microscopic identification/fingerprint/content determination/quality control分类
医药卫生引用本文复制引用
黎雪,聂雨杉,马雪,陆苑,杨畅,李勇军,王永林..水冬瓜根及茎的质量控制方法研究[J].中国药房,2024,35(1):21-26,6.基金项目
国家自然科学基金委员会-贵州省人民政府联合基金项目(No.U1812403) （No.U1812403）
贵州省高层次创新型人才培养计划——"百"层次人才项目(No.黔科合平台人才[2016]5677) （No.黔科合平台人才[2016]5677）
