水冬瓜根及茎的质量控制方法研究OACSTPCD

OBJECTIVE To establish the quality control method for the roots and rhizoma of Toricellia angulata.METHODS The properties of the roots and rhizoma of T.angulata were observed and microscopic identification was conducted.The moisture,total ash,acid-insoluble ash and ethanol-soluble extract were examined according to the method stated in the 2020 edition of Chinese Pharmacopoeia(part Ⅳ).HPLC fingerprints of 11 batches of the roots and rhizoma of T.angulata were established,common peaks were identified and the similarity was evaluated by using the Similarity Evaluation System of Chromatographic Fingerprint of TCM(2012 edition).The contents of coniferin,syringin,chlorogenic acid,(+)-syringaresinol-O-β-D-glucopyranoside and syringaresinol were determined by HPLC.RESULTS The properties and microscopic identification of the roots and rhizoma of T.angulata were obvious.The average contents of moisture,total ash,acid-insoluble ash and ethanol-soluble extract were 7.54%,2.18%,0.15%and 7.81%,respectively.There were 16 common peaks marked in the HPLC fingerprints of 11 batches of the roots and rhizoma of T.angulata,with similarities of 0.856-0.960;five of them were identified,such as coniferin,syringin,chlorogenic acid,(+)-syringaresinol-O-β-D-glucopyranoside and syringaresinol.The contents of the above five components were 0.047 2-0.401 6,0.836 8-8.697 9,1.245 3-10.950 0,0.139 0-0.437 8 and 0.016 4-0.635 3 mg/g,respectively.CONCLUSIONS The established method is stable and accurate,which can be used for the quality control of the roots and rhizoma of T.angulata.It is preliminarily proposed that the moisture in the roots and rhizoma of T.angulata is not more than 11.0%,the total ash is not more than 4.0%,the ethanol-soluble extract is not less than 5.0%,the contents of coniferin,syringin,chlorogenic acid,(+)-syringaresinol-O-β-D-glucopyranoside and syringaresinol are not less than 0.04,0.83,1.24,0.13,0.01 mg/g,respectively.