中国农业科技导报2024，Vol.26Issue(1)：18-27,10.DOI:10.13304/j.nykjdb.2023.0160
毕赤酵母外源蛋白分泌及折叠途径的改良进展
Improvement of Heterologous Protein Secretion and Folding Pathways of Pichia pastoris
摘要
Abstract
Most of the active proteins and industrial enzymes commonly used in industry,agriculture and medicine are obtained through heterologous expression systems.Pichia pastoris is one of the excellent hosts for expressing heterologous proteins.This expression system has the advantages including good genetic stability,high protein expression and secretory level,post-translational modification system,and low cost etc.However,excessive aggregation of heterologous proteins usually leads to incorrect fold and ineffective secretion of the target proteins,thus decling protein expression level.This paper reviewed the progress in the study on exogenous protein expression in Pichia pastoris by engineering the secretion and folding pathways such as signal peptide optimization,chaperone optimization and fusion protein expression.关键词
毕赤酵母/分泌折叠途径/分泌信号肽/分子伴侣/融合表达Key words
Pichia pastoris/secretion and folding pathways/signal peptide/molecular chaperones/fusion expression分类
农业科技引用本文复制引用
路泽群,刘宁,张红莲,王苑,黄火清..毕赤酵母外源蛋白分泌及折叠途径的改良进展[J].中国农业科技导报,2024,26(1):18-27,10.基金项目
国家重点研发计划项目(2021YFC2103002) （2021YFC2103002）
财政部和农业农村部现代农业产业技术体系建设项目(CARS-41). （CARS-41）
