中国农业科技导报2024，Vol.26Issue(1)：70-77,8.DOI:10.13304/j.nykjdb.2022.0561
磷恢复对磷饥饿铜绿微囊藻光合色素和部分抗氧化酶活性的影响
Effects of Phosphorus Recovery on Photosynthetic Pigments and Some Antioxidant Enzymes Activities of Phosphorus Starved Microcystis aeruginosa
摘要
Abstract
In order to explore the effects of phosphorus starvation and phosphorus recovery on the physiological indexes of Microcystis aeruginosa,its cells were treated with phosphorus starvation for 7 d and then phosphorus recovery was carried out.The cell density,the contents of chlorophyll a(chl-a),carotenoids,phycocyanin(PC),allophycocyanin(APC),phycoerythrin(PE),malondialdehyde(MDA),hydrogen peroxide(H2O2)and superoxide dismutase(SOD)activity were determined.The results showed that the density of M.aeruginosa was 2.54×107 cell·mL-1,significantly lower than that of the control group(3.11×107 cell·mL-1).After 144 h of phosphorus recovery,the algae density in the treatment group was 4.05×107 cell·mL-1,significantly lower than that of the control group(4.32×107 cell·mL-1).The contents of chl-a,carotenoids and PC,APC,PE showed a trend of gradual increase,which were 5.96、1.44 μg·mL-1 and 0.031,0.02,0.065 mg·L-1 at 144 h.The contents of MDA,H2O2 and the activity of SOD all increased first and then decreased,and reached the maximum value at 48 h,which increased by 36.2%,47.7%and 51.1%,respectively,compared with the control group.In conclusion,the cell density and the contents of Chla,carotenoid,phycobiliprotein of M.aeruginosa increased after phosphorus recovery,but it was difficult to recover to control level.The changes of MDA,H2O2 contents and SOD activity indicated that M.aeruginosa cells were damaged by oxidation and the cell membrane system was damaged by phosphorus starvation and phosphorus recovery.关键词
磷恢复/磷饥饿/铜绿微囊藻/光合色素/藻胆蛋白/抗氧化酶Key words
phosphorus recovery/phosphorus starvation/Microcystis aeruginosa/photosynthetic pigments/phycobili protein/antioxidant enzymes分类
农业科技引用本文复制引用
贾滢暄,张树林,张达娟,戴伟,毕相东..磷恢复对磷饥饿铜绿微囊藻光合色素和部分抗氧化酶活性的影响[J].中国农业科技导报,2024,26(1):70-77,8.基金项目
国家自然科学基金项目(32172978) （32172978）
天津市淡水养殖产业技术体系创新团队项目(ITTFRS2021000-009) （ITTFRS2021000-009）
中央引导地方(天津)科技发展专项(21ZYCGSN00500) （天津）
天津市教委科研计划项目(2020ZD06,2021KJ110) （2020ZD06,2021KJ110）
天津市高等学校创新团队基金项目(TD13-5089) （TD13-5089）
甘肃省科技计划项目民生科技专项(21CX6NP223). （21CX6NP223）
