中国中医药信息杂志2024，Vol.31Issue(1)：78-83,6.DOI:10.19879/j.cnki.1005-5304.202304186
麦粒灸对坐骨神经损伤大鼠脊髓组织TLR4/MyD88/NF-κB信号通路表达的影响
Effects of Wheat Grain Moxibustion on the Expression of TLR4/MyD88/NF-κB Signaling Pathway in Spinal Cord Tissue of Rats with Sciatic Nerve Injury
摘要
Abstract
Objective To observe the effects of wheat grain moxibustion for"Huantiao"on sciatic nerve function,pathological morphology of sciatic nerve stem and expressions of TLR4/MyD88/NF-κB signaling pathway expression in spinal cord tissue of rats with sciatic nerve injury(SNI);To explore the possible mechanism of wheat grain moxibustion for the treatment of SNI.Methods Totally 24 SD male rats were randomly divided into blank group,sham-operation group,model group and wheat grain moxibustion group,with 6 rats in each group.The model group and the wheat grain moxibustion group used a rat model with sciatic nerve clamping injury.From the 7th day after modeling,the rats were treated with moxibustion on the affected side of"Huantiao"for 6 strokes each time,once a day,for consecutive 10 days.The sciatic nerve function index(SFI)of rats on the 7th day after modeling and after intervention were observed,mechanical withdraw threshold(MWT)in rats were measured using a fiber optic pain gauge,ELISA was used to detect NO and iNOS content in spinal cord tissue,HE staining was used to observe the morphology of sciatic nerve stem,the expression of TLR4,NF-κBp65,p-NF-κBp65,MyD88,IκBα and p-IκBα in spinal cord tissue were detected by Western blot.Results Compared with the sham-operation group,the SFI and MWT of the rats in the model group significantly decreased(P<0.01),the arrangement of nerve fibers in sciatic nerve stem was disordered,with a significant increase in the number of Schwann cells and a large number of vacuolar degeneration,the content of NO,iNOS and the expression of TLR4,p-NF-κBp65,MyD88,p-IκBα protein in spinal cord tissue significantly increased(P<0.01).Compared with the model group,the SFI and MWT of the rats in the wheat grain moxibustion group increased significantly(P<0.01),the damage of sciatic nerve stem was reduced,with orderly cell arrangement,a decrease in the number of Schwann cells,and a decrease in axonal demyelination and cellular vacuolar degeneration,the content of NO,iNOS and the expression of TLR4,p-NF-κBp65,MyD88,p-IκBα in spinal cord tissue significantly decreased(P<0.05).Conclusion Wheat grain moxibustion for"Huantiao"can down-regulate TLR4,p-NF-κBp65,MyD88 and p-IκBα protein expressions in spinal cord tissue of SNI rats,reduce the secretion of NO and iNOS,thereby relieve pain and damaged nerve tissue inflammation response.关键词
麦粒灸/坐骨神经损伤/神经病理性疼痛/TLR4/MyD88/NF-κB信号通路/大鼠Key words
wheat grain moxibustion/sciatic nerve injury/neuropathic pain/TLR4/MyD88/NF-κB signaling pathway/rats分类
中医学引用本文复制引用
苏虹,张熙,李竞,郭彦君,粟胜勇,羊璞,王琼潇,谢彩云..麦粒灸对坐骨神经损伤大鼠脊髓组织TLR4/MyD88/NF-κB信号通路表达的影响[J].中国中医药信息杂志,2024,31(1):78-83,6.基金项目
国家自然科学基金(82160934、81960895) （82160934、81960895）
广西中医药大学桂派杏林拔尖人才项目(2022C017) （2022C017）
广西中医药重点学科建设项目(GZXK-Z-20-38) （GZXK-Z-20-38）
