中国中医药信息杂志2024，Vol.31Issue(1)：91-96,6.DOI:10.19879/j.cnki.1005-5304.202303041
补肾助孕方调节线粒体功能对子宫内膜血管生成的影响
Effects of Bushen Zhuyun Prescription on Endometrial Angiogenesis by Regulating Mitochondrial Function
摘要
Abstract
Objective To observe the effects of Bushen Zhuyun Prescription on regulating mitochondrial function and endometrial angiogenesis;To explore its mechanism of improving endometrial receptivity.Methods The mouse model of controlled ovarian hyperstimulation(COH)was established,and the mice were randomly divided into normal group,model group,and Bushen group,with 20 mice in each group.Bushen group received Bushen Zhuyun Prescription for gavage for 11 d,and the normal group and model group received normal saline for gavage.The number of embryo implantation was counted,the endometrial morphology was observed by HE staining,α-smooth muscle actin(α-SMA)expression was observed by immunofluorescence staining.Human endometrial microvascular endothelial cells(HEMECs)were cultured in vitro,they were divided into control group,VEGFA group,Bushen group and VEGFA + Bushen group,and were intervened with VEGFA and/or Bushen Zhuyun Prescription medicated serum.The activities of mitochondrial respiratory chain complexes Ⅰ-Ⅳ,the content of ATP,the expression of PCNA and Caspase-3 were detected.Results Animal experiment showed that,compared with the normal group,the number of embryo implantation in model group significantly decreased(P<0.05),α-SMA protein expression in endometrial tissue significantly decreased(P<0.05);compared with the model group,the number of embryo implantation in Bushen group significantly increased(P<0.05),α-SMA protein expression in endometrial tissue significantly increased(P<0.05).Cell experiment showed that,Bushen Zhuyun Prescription medicated serum could increase the activity of mitochondrial respiratory chain complexes Ⅰ-Ⅳ and ATP content in HEMECs,promote PCNA protein expression,and inhibit Caspase-3 protein expression(P<0.05,P<0.01).Conclusion Bushen Zhuyun Prescription can promote endometrial angiogenesis through improving mitochondrial function.关键词
补肾助孕方/肾主生殖/线粒体/血管生成/子宫内膜容受性/小鼠Key words
Bushen Zhuyun Prescription/kidney governing reproduction/mitochondria/angiogenesis/endomentrial receptivity/mice分类
医药卫生引用本文复制引用
周玉玲,张静蕾,曹璨,孙莹,牛若冰,张英美,胡书婵,罗琳,贺明..补肾助孕方调节线粒体功能对子宫内膜血管生成的影响[J].中国中医药信息杂志,2024,31(1):91-96,6.基金项目
国家自然科学基金(82074247) （82074247）
河北省自然科学基金(H2021423031) （H2021423031）
河北省研究生创新项目(CXZZSS2022103) （CXZZSS2022103）
河北省大学生创新创业训练计划项目(S202114432008) （S202114432008）
河北省大中学生科技创新能力培育专项(22E50130D、22E50135D) （22E50130D、22E50135D）
