中国中医药信息杂志2024，Vol.31Issue(1)：104-109,6.DOI:10.19879/j.cnki.1005-5304.202211552
寿胎丸调控组蛋白修饰对复发性流产小鼠子宫内膜蜕膜化的影响
Effects of Shoutai Pills in Regulating Histone Modification on Endometrial Decidualization of Mice with Recurrent Spontaneous Abortion
摘要
Abstract
Objective To observe the effect of Shoutai Pills on endometrial decidualization of mice with recurrent spontaneous abortion(RSA);To explore its possible mechanism in the treatment of RSA based on histone modification.Methods Totally 40 female CBA/J mice were divided into normal group,model group,Shoutai Pills low-dosage group(7.5 g/kg),Shoutai Pills high-dosage group(15 g/kg)and dydrogesterone group(3 mg/kg).The normal group were co housed with BALB/C male mice,while the other groups were co housed with DBA/2 male mice to establish an RSA mouse model.After modeling,the administration groups were given corresponding medication solution by gavage,while the normal group and model group were given equal volume of pure water by gavage for 10 consecutive days.The embryo condition was observed and the embryo loss rate was calculated,ELISA was used to detect serum prolactin(PRL)content,HE staining was used to observe the morphological changes of decidual tissue,RT-PCR was used to detect PRL mRNA expression in decidual tissue,Western blot was used to detect the protein expressions of H4ac,H3K27ac,H3K27me3.Results Compared with the normal group,the model group mice showed a significant increase in embryo loss rate,a significant decrease in serum PRL content,disordered arrangement of decidual cells,and extensive bleeding and necrosis;the expression of PRL mRNA and protein in decidual tissue significantly decreased,the protein expressions of H4ac and H3K27ac significantly decreased,while the expression of H3K27me3 protein significantly increased,with statistical significance(P<0.05).Compared with the model group,the embryo loss rate of Shoutai Pills low-and high-dosage groups and the dexamethasone group significantly decreased,the serum PRL content significantly increased,tightly arranged decidual cells,reduced necrosis,and intact glands;the expression of PRL mRNA and protein in decidual tissue of mice in Shoutai Pills high-dosage group and the dexamethasone group significantly increased,the protein expressions of H4ac and H3K27ac significantly increased,the expression of H3K27me3 protein significantly decreased,with statistical significance(P<0.05).Conclusion Shoutai Pills can promote endometrial decidualization in RSA mice,which is related to the changes of histone modification in endometrial stromal cells.关键词
寿胎丸/复发性流产/蜕膜组织/组蛋白修饰/小鼠Key words
Shoutai Pills/recurrent spontaneous abortion/endometrial decidualization/histone modification/mice分类
医药卫生引用本文复制引用
靳梓琪,任守杨,王天一,马珺卓,王子璐,张铭娜,张宇..寿胎丸调控组蛋白修饰对复发性流产小鼠子宫内膜蜕膜化的影响[J].中国中医药信息杂志,2024,31(1):104-109,6.基金项目
国家自然科学基金联合基金(U21A20403) （U21A20403）
国家自然科学基金面上项目(82074247) （82074247）
河北省自然科学基金(H2021423031) （H2021423031）
河北省中医药管理局科研计划(2022182) （2022182）
河北省研究生创新项目(CXZZSS2022103) （CXZZSS2022103）
河北省大学生科技创新能力培育专项(22E50130D、22E50135D) （22E50130D、22E50135D）
国家大学生创新创业训练计划(202114432012) （202114432012）
